A: Specific staining of ATG5 expressed in human neuroblastoma SH-SY5Y cell line by Flow Cytometry using cat # M-882-100. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to ATG5 (2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 dilution), incubation for 20 minutes in dark at room temperature. Negative control: Non-specific Control IgG, clone X63 (cat # M-1249-200, black dashed). Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Western blot detection of GST tagged recombinant human ATG5. Note the GST tag alone is 26 kDa. C: Sandwich ELISA using anti-ATG5 monoclonal antibody as a capture antibody. The detection limit for GST tagged recombinant ATG5 protein is 0.1 ng/ml.