Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.25 µg/mL is recommended for WB. Human BIN1 has a predicted length of 593 aa and MW of 77 kDa. A concentration of 0.5 µg/mL is recommended for IHC to detect BIN1 in formalin/acetone fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMyc box-dependent-interacting protein 1 (BIN1)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; chicken; pig;
Target Host SpeciesHuman
Species ReactivityChicken, Human, Mouse, Pig, Rat
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone NameBN-1
ConjugateUnconjugated
Immunogen DescriptionRecombinant polypeptide corresponding to amino acids 189-398 from human BIN1.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesAMPHL; Amphiphysin-like protein; SH3P9;
Scientific BackgroundBox-dependent myc interacting protein 1 (BIN1) interacts with and inhibits the oncogenic activity of the myc oncoprotein and thus may act as a tumour suppressor.