Product DescriptionMouse anti-Calcium/calmodulin-dependent protein kinase 1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB and both direct and sandwich ELISA. This antibody has a detection limit of 0.3ng/mL for recombinant GST tagged CaM kinase 1 when used in sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetCalcium/calmodulin-dependent protein kinase 1
SpecificityThe specificity of this antibody has been confirmed by WB and ELISA against the recombinant GST tagged CaM Kinase 1 protein. No other species have been tested yet.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant protein of human CaM kinase 1 (amino acids 271 to 370) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesCalcium/calmodulin-dependent protein kinase type 1; CaM-KI; CaM kinase I alpha; CAMKI-alpha; CAMK1
CaM kinase I belongs to a calcium-triggered signalling cascade involved in trasncriptional regulation, hormone production, translational regulation, regulation of actin filament organisation and neurite outgrowth. CaM kinase I is involved in calcium-dependent activation of the ERK pathway. It phosphorylates proteins with the substrate consensus sequence [MVLIF}-x-R-x(2)-[ST]-x(3)-[MVLIF]. It is activated by calcium ions or calmodulin. It reaches its maximum catalytic activity when phosphorylated by CAMKK1 or CAMKK2. CaM kinase I has a length of 370 amino acids (unprocessed precursor) and a predicted molecular weight of 41.3KDa. It is a monomer that exists in many tissues and is located within the cytoplasm or nucleus. It belongs to the protein kinase superfamily, the CAMK Ser/Thr protein kinase family and the CaMK subfamily.
Mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number M-810-100, detects a band of molecular weight 42KDa in human promyelocytic leukemia (HL-60) cell lysate under denaturing conditions for WB.
Sandwhich ELISA using mouse monoclonal antibody to human CaM kinase 1 [3G1], catalogue number M-810-100. This antibody has a detection limit of 0.3ng/mL for recombinant GST tagged CaM kinase 1 protein.