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RNA-binding protein Musashi homolog 1, Mouse Monoclonal Antibody
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Catalog Number
M-1720
- Product Name RNA-binding protein Musashi homolog 1, Mouse Monoclonal Antibody
- Product Description Mouse anti-RNA-binding protein Musashi homolog 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
- Alternative Names RNA-binding protein Musashi homolog 1; Musashi1; MSI1
- Application(s) ELISA, ICC, IHC-Frozen, WB
- Antibody Host Mouse
- Antibody Type Monoclonal
- Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
- Species Reactivity Human
- Immunogen Description Full length recombinant human Musashi 1 protein (1-87) with a GST tag.
- Conjugate Unconjugated
- Purity Description Protein G purified immunoglobulin
- Regulatory Status For research use only.
Product Info
- Product Description Mouse anti-RNA-binding protein Musashi homolog 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
- Application(s) ELISA, ICC, IHC-Frozen, WB
- Application Details This antibody is recommended for WB, IHC, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. The antibody has been used successfully on formalin fixed, paraffin embedded tissue. It is necessary to treat the paraffin sections by placing them in 1X Citrate Buffer (pH 6.0) then placing in a Pressure Cooker at 125ºC for 4 minutes then at 90ºC for 45 min. The tissue may also be treated by placing it in 1X Citrate Buffer (pH 6.0) then microwaving at 750W for 20 minutes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
- Target RNA-binding protein Musashi homolog 1
- Specificity Specificity has been confirmed by WB and direct ELISA against the antigen.
- Target Host Species Human
- Species Reactivity Human
- Antibody Host Mouse
- Antibody Type Monoclonal
- Antibody Isotype IgG2a
- Clone Name 3F2
- Conjugate Unconjugated
- Immunogen Description Full length recombinant human Musashi 1 protein (1-87) with a GST tag.
- Purity Description Protein G purified immunoglobulin
- Format Lyophilized from PBS pH 7.4.
- Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
- Storage Instructions After reconstitution keep aliquots at -20°C for up to six months. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt (unopened vial).
- Alternative Names RNA-binding protein Musashi homolog 1; Musashi1; MSI1
- Uniprot Number O43347
- Uniprot Number/Name O43347 (MSI1H_HUMAN)
- Scientific Background Musashi 1 is an RNA binding protein that regulates the expression of target mRNAs at the translational level. It contains two conserved tandem RNA recognition motifs. Musashi 1 binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. It is detected in fetal kidney, brain, liver and lung and adult brain and pancreas. It is also detected in hepatoma cell lines.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 41116161
- Regulatory Status For research use only.
