Product DescriptionMouse anti-RNA-binding protein Musashi homolog 1 Monoclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, ICC, ELISA.
Application(s)ELISA, ICC, IHC-Frozen, WB
Application DetailsThis antibody is recommended for WB, IHC, sandwich ELISA and immunofluorescence. The recommended dilution for this antibody is 3 µg/mL for IHC and 10 µg/mL for immunofluorescence. The antibody has been used successfully on formalin fixed, paraffin embedded tissue. It is necessary to treat the paraffin sections by placing them in 1X Citrate Buffer (pH 6.0) then placing in a Pressure Cooker at 125ºC for 4 minutes then at 90ºC for 45 min. The tissue may also be treated by placing it in 1X Citrate Buffer (pH 6.0) then microwaving at 750W for 20 minutes. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetRNA-binding protein Musashi homolog 1
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen.
Target Host SpeciesHuman
Immunogen DescriptionFull length recombinant human Musashi 1 protein (1-87) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.4.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for up to six months. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesRNA-binding protein Musashi homolog 1; Musashi1; MSI1
Scientific BackgroundMusashi 1 is an RNA binding protein that regulates the expression of target mRNAs at the translational level. It contains two conserved tandem RNA recognition motifs. Musashi 1 binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U(1-3)AGU-3'. It may play a role in the proliferation and maintenance of stem cells in the central nervous system. It is detected in fetal kidney, brain, liver and lung and adult brain and pancreas. It is also detected in hepatoma cell lines.