Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 0.5-2.0 µg/mL is recommended for WB. Human myoglobin (precursor) has a predicted length of 154 residues and MW of 17 kDa. A concentration of 1.0-2.0 µg/mL is recommended to detect myoglobin in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Rat
Immunogen DescriptionPurified human skeletal muscle myoglobin
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundMyoglobin is a small heme containing protein that functions as a reserve supply of oxygen as well as facilitating the movement of oxygen within muscles. At least 3 isoforms of human Myoglobin are produced by alternative splicing.