A: Specific staining of NeuroD1 expressed in human neuroblastoma SH-SY5Y cell line by Flow Cytometry using cat # M-850-100. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to NeuroD1 (2 ug per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 dilution), 20 minutes in dark at room temperature. Negative control: Non-specific Control IgG, clone X63 (cat # M-1249-200, black dashed). Data and results were generated using Orflo MoxiflowTM instrument and protocols. B: Immunohistochemical detection of NeuroD1 on formalin fixed, paraffin-embedded, human ovary, clear cell carcinoma. Anti-NeuroD1 primary antibody was used at a concentration of 3 µg/mL. C: Western blot detection of NeuroD1 expression in human neuroblastoma cell lysate. D: Immunofluorescent detection of NeuroD1 in HeLa cells. Antibody concentration: 10 µg/mL.