Product DescriptionMouse anti-Neurturin Monoclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern blot (WB) at a suggested dilution of 1:5,000-1:10,000. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityHuman NTN (Neurturin)
Target Host SpeciesHuman
Immunogen DescriptionRecombinant human NRTN protein produced using CHO-based suspension cell line. Protein was purified from the cell culture supernatant.
Purity DescriptionProtein G purified IgG
FormatPBS pH 7.4, with 0.1% sodium azide
Storage InstructionsStore at -20°C or -70°C upon receipt. After opening divide antibody into smaller aliquots and store at -20°C or -70°C for up to six months. Avoid multiple freeze-thaw cycles as product degradation may result.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundNeurturin (NTN) is a member of the GDNF family of neurotrophic factors. This protein is a potent survival factor for several populations of central and peripheral neurons in mature and developing rodents. FUNCTION: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. SUBUNIT: Homodimer; disulfide-linked. SUBCELLULAR LOCATION: Secreted protein. DISEASE: Defects in NRTN are a cause of Hirschsprung disease (HSCR). In association with mutations of RET gene, and possibly with other loci, defects in NRTN are involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. SIMILARITY: Belongs to the TGF-beta family. GDNF subfamily.
Western-Blot detection of human NRTN expressed in CHO cells. Line 1. hNRTN-containing CHOEBNALT85 cell culture supernatant. Line 2. Negative control - CHOEBNALT85 cell culture supernatant. 5 µl of supernatant were loaded per line. Primary antibody dilution 1:5000 was used. Antibody recognizes protein dimeric, and monomeric variants.