Application DetailsThis antibody is recommended for WB, IHC and sandwich ELISA. The recommended dilution for this antibody is 3 µg/mL for IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant human Pleiotrophin (45-154) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Immunohistochemical detection of Pleiotrrophin on formalin fixed, paraffin-embedded, human cerebral cortex. Anti-Pleiotrophin primary antibody, catalogue number M-860-100, was used at a concentration of 3 µg/mL.
Western blot detection of Pleiotrophin in HeLa nuclear extract.