Protein S100-A1, Mouse Monoclonal Antibody

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Catalog Number
M-863

    Product Info

  • Product Name Protein S100-A1, Mouse Monoclonal Antibody
  • Product Description google Mouse anti-Protein S100-A1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Alternative Names S100 calcium-binding protein A1 S-100 protein alpha subunit; S-100 protein alpha chain; Roundabout homolog 1; S100A1; S100A
  • Application(s) ELISA, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Species Reactivity Human
  • Immunogen Description Partial recombinant human Protein S100-A1 (amino acids 1-75) with a GST tag.
  • Conjugate Unconjugated
  • Purity Description Protein G purified immunoglobulin
  • Regulatory Status For research use only.

    Specifications

  • Product Description Mouse anti-Protein S100-A1 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
  • Application(s) ELISA, WB
  • Application Details This antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Protein S100-A1
  • Specificity Specificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
  • Target Host Species Human
  • Species Reactivity Human
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG
  • Clone Name 1D5
  • Conjugate Unconjugated
  • Immunogen Description Partial recombinant human Protein S100-A1 (amino acids 1-75) with a GST tag.
  • Purity Description Protein G purified immunoglobulin
  • Format Lyophilized from PBS pH 7.2
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions After reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names S100 calcium-binding protein A1 S-100 protein alpha subunit; S-100 protein alpha chain; Roundabout homolog 1; S100A1; S100A
  • Uniprot Number P23297
  • Uniprot Number/Name P23297 (S10A1_HUMAN)
  • Scientific Background
    Protein S100-A1 is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Protein S100-A1 exists as a dimer of either two alpha chains, two beta chains or one alpha and one beta chain. It weakly binds calcium but binds zinc very tightly and distinct binding sites with different affinities exist for both ions on each monomer. This protein is highly prevalent in heart and to a lesser extent in skeletal muscle and brain.
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Western blot detection of GST tagged recombinant human Protein S100-A1. Note the GST tag alone is 26 kDa.

  • Sandwich ELISA using anti-Protein S100-A1 as a capture antibody. The detection limit for GST tagged recombinant Protein S100-A1 is 0.3 ng/mL.

    Citations & References