Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 4.0 µg/mL is recommended for WB. Human c-Myc (isoform 1) has a predicted length of 439 residues and a MW of 49 kDa. A concentration of 8.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMyc proto-oncogene protein
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionA synthetic peptide (AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA) corresponding to C terminal residues 408-439 of human Myc proto-oncogene protein (c-Myc).
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative Namesc-Myc; Transcription factor p64; MYC;
Scientific BackgroundMyc proto-oncogene protein (c-Myc) is a transcription factor with roles in cellular proliferation, differentiation, apoptosis and cell cycle progression. Mutations in the c-Myc gene and protein over-expression have been associated with a variety of hematopoietic tumors, leukemias and lymphomas.