Product DescriptionMouse anti-Secretogranin-5 Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application DetailsThis antibody is recommended for WB and direct ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human, mouse and rat. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionPartial recombinant human Secretogranin-5 (amino acids 59-160) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNeuroendocrine protein 7B2; Secretory granule endocrine protein I; Secretogranin V; Pituitary polypeptide; SCG5; SGNE1
Secretogranin-5 is an acidic protein of 186 amino aids and has an expression that seems to be restricted to neurons and endocrine cells. In the brain, it is most abundant in the supraoptic and paraventricular nuclei of the hypothalamus. It acts in neuroendocrine cells as a specific chaperone for proprotein convertase-2 (PC2) which is an important endoprotease involved in the processing of neuroendocrine precursors. It binds to inactive proPC2 in the ER and facilitates its movement toward the secretory pathway where proPC2 is proteolytically matured and activated. It also plays a role in regulating pituitary hormone secretion.