Left: Western blot detection of Tyrosine kinase receptor type B (TrkB) expression in human osterogenic sarcoma cell lysate. Right: Analysis of TrkB expression in human prostate cancer DU145 cell line by Flow Cytometry. DU145 cells washed twice using PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to TrkB (cat # M-853-100, 2 μg per ~10^6 cells) for 30 minutes on ice, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.