Application DetailsImmunohistochemistry (IHC), Immunocytochemistry (ICC) and Western Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Vinculin (isoform 2, Metavinculin) has a predicted length of 1,134 residues and a MW of 124 kDa. A concentration of 2.0-4.0 µg/mL is recommended to detect the protein in formalin/acetone fixed frozen tissues. A concentration of 2.0 µg/mL is recommended for IC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat; chicken;
Target Host SpeciesHuman
Species ReactivityChicken, Human, Mouse, Rat
Immunogen DescriptionPurified Vinculin from human uterus
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Vinculin staining in fixed and permeabilized normal human epidermal keratinocytes by Immunocytochemistry. Vinculin-IR (green) is observed in the cytoplasm, consistent with expected cellular localization. Blue: Nuclei. Magnification: 32X. Image courtesy of QBM Cell Science.
Vinculin staining (red) in human myotubes by Immunocytochemistry. Blue: Cell nuclei. Image courtesy of QBM Cell Science.
Staining of vinculin (green) in human renal proximal tubule cells by Immunocytochemistry with mouse antibody to vinculin (M-1228-100). Red: phalloidin. Blue: cell nuclein. Image courtesy of QBM Cell Science.