Left: Analysis of alpha-internexin expression in rat cerebellum with mouse antibody to alpha-internexin (1:5,000, green) by Immunohistochemistry. Co-staining was performed with chicken antibody to calretinin (C-1801-50, 1:2,000, red). IHC Method: Following transcardial perfusion of rat with 4% PFA, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The alpha-internexin antibody selectively stains neuronal processes, in particular parallel fibers, the axons of granule cells. Calretinin antibody stains interneurons predominantly in the molecular layer of the cerebellum. Right: Western blot analysis of alpha-internexin expression in tissue lysates using mouse antibody to alpha-internexin (1:10,000, green):  protein standard,  rat brain,  rat spinal cord,  mouse brain,  mouse spinal cord,  pig spinal cord,  cow spinal cord. Alpha-internexin protein migrates on the gel with apparent molecular weight of 64 to 66 kDa with slight variability among species.