Left: Detection of mCherry protein (red) in transfected HEK293 cells by Immunocytochemistry. The cells were stained with anti-mCherry antibody in the green channel. Transfected cells appear yellow, showing overlap of the transfected mCherry protein and the anti-mCherry antibody. Untransfected HEK293 cells do not express mCherry and do not stain with the antibody, but their nuclei can be visualized using a DNA stain (blue). Right: Western blot analysis of HEK293 cell lysates, and recombinant protein solutions using mouse antibody to mCherry (green, 1:1,000).  protein standard,  HEK293 control cells,  HEK293 cells transfected with mCherry-HA construct,  mCherry recombinant protein,  GFP recombinant protein, and  HEK293 transfected with GFP construct. The major band at about 30 kDa corresponds to mCherry protein. The antibody does not react with GFP protein. The same blot was simultaneously probed an antibody to HSP60 (red, lanes 2-6).