Application DetailsThis antibody is recommended for WB and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetMelanoma growth stimulatory activity (MGSA)
SpecificityThe specificity of this antibody has been confirmed by WB and ELISA against the GST tagged recombinant human MSGA protein. Human. Other species have not been tested.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant protein of human MGSA (amino acids 36 to 107) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.4
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesMelanoma growth stimulatory activity; growth-regulated alpha protein; GRO-alpha(1-73); C-X-X motif chemokine 1; neutrophil-activating protein 3; NAP-3; CXCL1
MGSA belongs to the intercrine alpha (chemokine CXC) familly. In this family, the first two of the four conserved residues are separated by a single amino acid. MGSA has chemotactic activity for neutrophils and may play a role in inflammation. MGSA is secreted and subsequently proteolytically processed at the N-terminus by peripheral blood monocytes to form GRO-alpha(4-73), GRO-alpah(5-73) and GRO-alpha(6-73). In vitro, these processed forms show a 30 fold increase in chemotactic activity. The unprocessed precursor has a length of 107 amino acids and a predicted molecular weight of 11.3KDa.