E18 hippocampal neurons were grown for seven days. The neurons were fixed and immunostained with M-1703-100 (red channel). The cells were also stained in green with C-1377-50, our chicken antibody to alpha Internexin and DNA (blue). M-1703-100 stains the neuronal perikarya and process strongly, and does not stain non neuronal cells in these cultures. The alpha internexin antibody stains intermediate or 10nm filament bundles in the cytoplasm of these cells.
Left: Analysis of MAP-tau expression (green) in cortical neuron-glial culture from E20 rat by Immunocytochemistry. Mouse antibody to MAP-tau was used at 1:1,000 dilution. Co-staining was performed with chicken antibody to MAP2 (C-1382-50, red, 1:5,000). Blue: DAPI nuclear stain. The MAP-tau antibody stains perikarya, dendrites and axons of neurons, while the MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both proteins. Right: Western blot analysis of MAP-tau expression in tissue lysates using mouse antibody to MAP-tau (green, 1:2,000).  protein standard,  rat brain,  rat spinal cord,  mouse brain,  mouse spinal cord. Tau protein is expressed as up to 9 different isoforms of different molecular weights, therefore, it appears as multiple closely spaced bands in the range from 48 kDa to 67 kDa in the CNS (lanes 2-4). In the PNS, additional higher molecular weight bands are observed (lane 5).