Product DescriptionMouse anti-Nebulin Monoclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human Nebulin (P20929-1) has a predicted length of 6,669 residues and a MW of 773 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; chicken; frog;
Target Host SpeciesChicken
Species ReactivityChicken, Frog, Human, Mouse, Rat
Immunogen DescriptionFull length native protein purified from Chicken breast
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundNebulin is a component of the cytoskeletal matrix with possible roles in maintaining the structural integrity of sarcomeres and the membrane system associated with the myofibrils. The Nebulin gene encodes numerous splice variants in a manner that is tissue, species and developmental stage specific.