Left: Analysis of nuclear pore complex (NPC) expression in HeLa cells by Immunocytochemistry. Cells were stained with pan-specific mouse anti-NPC antibody (red, 1:100), and co-stained with chicken antibody to vimentin (C-1409-50, green, 1:10,000). Blue: DAPI nuclear stain. The NPC antibody reveals strong granular staining of the nuclei corresponding to the localization of NPC, while the vimentin antibody labels intermediate filaments in these cells. Right: Western blot analysis of cell lysates for NPC expression (green, lanes 2-7, 1:100), using cytosol- or nuclear-enriched fractions.  protein standard,  HEK293 cytosol,  HEK293 nuclear,  NIH-3T3 cytosol,  NIH-3T3 nuclear,  HeLa cytosol, and  HeLa nuclear fraction lysate. The band at about 68 kDa represents a currently unidentified NPC protein which is detected predominantly in the nuclear enriched fractions of all cell lines.