NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (FITC)

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Catalog Number
M-016-FT
Discontinued

    Product Info

  • Product Name NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody (FITC)
  • Product Description google
    Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for IHC-Frozen, ICC, FC.
  • Replacement Product

    Please refer to abc M-006-100 : NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody, Clone MC192, which is the unconjugated version of M-016-FT-100. 

  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Application(s) FC, ICC, IHC-Frozen
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. Reacts with rat. Does not react with mouse or human p75 NGFR
  • Species Reactivity Rat
  • Immunogen Description Rat p75NTR
  • Conjugate FITC
  • Purity Description Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    Mouse anti-p75 neurotrophin receptor (p75NTR) Monoclonal Antibody (FITC), suitable for IHC-Frozen, ICC, FC.
  • Related Products Non-specific Control IgG, Mouse Monoclonal Antibody (FITC)
  • Replacement Product

    Please refer to abc M-006-100 : NGFR/p75 neurotrophin receptor (p75NTR), Mouse Monoclonal Antibody, Clone MC192, which is the unconjugated version of M-016-FT-100. 

  • Application(s) FC, ICC, IHC-Frozen
  • Application Details Immunohistochemistry, immunofluorescence, flow cytometry, NGF receptor p75 dynamics, retrograde transport studies, study of intracellular trafficking. Suggested working dilutions: For immunohistochemistry a concentration of 1-2 µg/mL is recommended. The antibody is not appropriate for Western Blots. The recommended concentration for FACS is 20 µg/mL and at least 1 in 5000 dilution is recommended for 1-site ELISA. Optimal working dilution should be determined by the end user. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984). Biosensis recommends optimal dilutions/concentrations should be determined by the end user. The FITC version of MC192 is primarily targeted for FACS or IF applications on live or lightly fixed cells. Antibody will not work in traditional formalin fixed tissues.
  • Target NGFR/p75 neurotrophin receptor (p75NTR)
  • Specificity MC192 recognizes the extracellular domain of the neurotrophin receptor p75NTR in rat. Reacts with rat. Does not react with mouse or human p75 NGFR
  • Target Host Species Rat
  • Species Reactivity Rat
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Antibody Isotype IgG1
  • Clone Name MC192
  • Conjugate FITC
  • Ex/Em Max Excitation maximum: 490 nm. Emission maximum: 525 nm.
  • Immunogen Description Rat p75NTR
  • Purity Description Immunoglobulin (IgG1) was purified using Protein G column (Amersham Pharmacia), polished with Sephacryl 200HR (Amersham Pharmacia) in PBS. The antibody was then conjugated to Fluorescein isomer 1 (FITC, Sigma). A minimum fluorescein: protein ratio of 3:1 is guaranteed. The conjugate was purified via gel filtration using a G25 fine grain gel in 10 mMTris/50mM NaCl solution.
  • Format Liquid
  • Storage Instructions The antibody conjugate can be stored at 2-8°C for up to 4 months with the addition of appropriate antibacterial agent.
  • Batch Number Please see item label.
  • Expiration Date 4 months after date of receipt (unopened vial)
  • Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; CD271
  • Uniprot Number P07174
  • Uniprot Number/Name P07174 (TNR16_RAT)
  • Scientific Background Monoclonal antibody MC192 against the rat low affinity nerve growth factor receptor (p75NTR) is derived from the fusion of Sp2/0-Ag 14 myeloma cells with mouse immune splenocytes. MC192 monoclonal antibody was originally generated by Chandlers et al. p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. MC192 has a potential use as the ligand for gene delivery into p75NTR-expressing rat cells via a receptor-mediated mechanism. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Immunofluorescent staining of rat basal forebrain cholinergic neurons using MC192-FITC conjugated antibodies, following intraventricullar delivery (5 ยตg). Animals were sacrificed and fixed 72hr following intraventricular injections, and processed for microscopy.