Parkinson disease protein 7 (PARK7), Mouse Monoclonal Antibody

Product Info

Product Name Parkinson disease protein 7 (PARK7), Mouse Monoclonal Antibody
Product Description google Mouse anti-Parkinson disease protein 7 (PARK7) Monoclonal Antibody (Unconjugated), suitable for WB, ICC..
Alternative Names Oncogene DJ1; Parkinson disease protein 7; PARK7; DJ-1
Application(s) ICC, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry. Does not react with rat and mouse DJ-1 protein on western blots.
Species Reactivity Human
Immunogen Description Full length recombinant human DJ-1 expressed in and purified from E. coli.
Conjugate Unconjugated
Purity Description Protein G purified IgG
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Parkinson disease protein 7 (PARK7) Monoclonal Antibody (Unconjugated), suitable for WB, ICC..
Application(s) ICC, WB
Application Details WB, ICC. Suggested dilution of at least 1:500 for ICC. Dilutions of 1:5,000 or lower is recommended for WB. This antibody reveals a prominent ~21 kDa band and stains mainly in cytoplasm of tissue culture cells. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Parkinson disease protein 7 (PARK7)
Specificity The antibody reacts with a 21 kDa band by Western blot on whole HeLa cell lysate. It has also been used successfully for immunocytochemistry. Does not react with rat and mouse DJ-1 protein on western blots.
Target Host Species Human
Species Reactivity Human
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG1, kappa
Clone Name 4H4
Conjugate Unconjugated
Immunogen Description Full length recombinant human DJ-1 expressed in and purified from E. coli.
Purity Description Protein G purified IgG
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 uL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Oncogene DJ1; Parkinson disease protein 7; PARK7; DJ-1
Uniprot Number Q99497
Uniprot Number/Name Q99497 (PARK7_HUMAN)
Scientific Background Protein DJ-1 has many roles including protecting cells against oxidative stress and cell death (Ref: SwissProt). Mutations in the DJ-1 gene have been associated with rare forms of autosomal recessive early-onset Parkinson's disease.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

HeLa cells stained with Mouse monoclonal antibody to DJ-1 M-1573-100 (green), and Chicken polyclonal antibody to Vimentin C-1409-50 (red) and DNA (blue). M-1573-100 antibody reveals strong cytoplasmic staining for DJ-1.
Whole HeLa cell lysate stained with mouse anti-DJ1 antibody. The antibody recognizes the ~21 kDa protein.

Western blot analysis of whole brain and cell lysates using mouse antibody to DJ1 (green, 1:5,000). [1] protein standard, [2] rat brain, [3] mouse brain, [4] NIH-3T3, [5] HeLa, [6] HEK293, and [7] C6 cells. The antibody detects DJ1 protein with apparent molecular weight of 21 kDa, but only in human cell lines, since it does not recognize the rat or mouse DJ1 protein. The blot was simultaneously probed with chicken antibody to vimentin (C-1409-50, red, 1:5,000), revealing a single band at about 50 kDa (lanes 2-7).

Citations & References

General References Nagakubo D, Taira T, Kitaura H, Ikeda M, Tamai K, Iguchi-Ariga SM and Ariga H DJ-1, a novel oncogene which transforms mouse NIH3T3 cells in cooperation with ras. Biochem. Biophys. Res. Commun. 231, 509-513 (1997).
Bonifati V, Rizzu P, van Baren, M.J., Schaap O, Breedveld GJ, Krieger E, Dekker MC, Squitieri F, Ibanez P, Joosse M et al. Mutations in the DJ-1 gene associated with autosomal recessive early-onset Parkinsonism. Science, 299, 256-259 (2003).
Xu J, Zhong N, Wang H, Elias JE, Kim CY, Woldman I, Pifl C, Gygi SP, Geula C, Yankner BA. The Parkinson's disease-associated DJ-1 protein is a transcriptional co-activator that protects against neuronal apoptosis. Hum Mol Genet. 14 (9):1231-41 (2005).
Yokota T, Sugawara K, Ito K, Takahashi R, Ariga H. And Mizusawa, H. Down regulation of DJ-1 enhances cell death by oxidative stress, ER stress, and proteasome inhibition. Biochem. Biophys. Res. Commun., 312, 1342-1348 (2003).
Taira T, Saito Y, Niki T, Iguchi-Ariga SM, Takahashi K and Ariga H. DJ-1 has a role in antioxidative stress to prevent cell death. EMBO Rep., 5, 213-218 (2004).
Bonifati V, Oostra BA. and Heutink, P. Linking DJ-1 to neurodegeneration offers novel insights for understanding the pathogenesis of Parkinson's disease. J.Mol,Med.82,163-174 (2004).