Peripherin, Mouse Monoclonal Antibody

Product Info

Product Name Peripherin, Mouse Monoclonal Antibody
Product Description google Mouse anti-Peripherin Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Alternative Names Peripherin; Prph; Prph1;
Application(s) ICC, IHC-Frozen, WB
Antibody Host Mouse
Antibody Type Monoclonal
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. Human, mouse, feline. Predicted to react with other mammalian tissue.
Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
Immunogen Description Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Conjugate Unconjugated
Purity Description Affinity purified.
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Peripherin Monoclonal Antibody (Unconjugated), suitable for WB, ICC, IHC-Frozen.
Application(s) ICC, IHC-Frozen, WB
Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). A concentration of 0.5 - 2 µg/mL is recommended for WB. A concentration of 1-5 µg/mL is recommended for IC and IH. This antibody performs well on aldehyde fixed tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Peripherin
Specificity The specificity of this antibody has been confirmed by WB. This antibody detects ~57 kDa Peripherin protein. Human, mouse, feline. Predicted to react with other mammalian tissue.
Target Host Species Rat
Species Reactivity Cat, Human, Mouse, Other Mammals (Predicted), Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG
Clone Name 7C5
Conjugate Unconjugated
Immunogen Description Recombinant full length rat Peripherin protein expressed in and purified from E.coli
Purity Description Affinity purified.
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water, to achieve an antibody concentration of 1 mg/mL. Centrifuge briefly to remove any insoluble material.
Storage Instructions Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Peripherin; Prph; Prph1;
Uniprot Number P21807
Uniprot Number/Name P21807 (PERI_RAT)
Scientific Background Peripherin is a class-III neuronal intermediate filament protein found in certain classes of neuron, most of which are located in the peripheral nervous system.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Mixed neuron/glia cultures from newborn rat brain stained with Mouse monoclonal antibody to Peripherin [7C5] (green) and Rabbit polyclonal antibody to NF-L R-1392-50 (red channel). A class of large neurons, like the one in the middle of this image, contain peripherin, while the majority of neurons and their processes contain NF-L and not Peripherin. Interestingly, the periperin positive cells often contain a cytoplasmic inclusion next to the nucleus which stains for both Peripherin and NF-L, and so appears golden in this kind of image. The blue channel reveals the localization of DNA.

Citations & References

Specific References Sekerkova G. et al (2008) Espin actin-cytoskeletal proteins are in rat type I spiral ganglion neurons and include splice-isoforms with a functional nuclear localization signal. J Comp Neurol. 2008 Aug 20;509(6):661-76.