Application DetailsWestern Blotting (WB). A concentration of 1.0-2.0 µg/mL is recommended for WB. Human M-phase inducer phosphatase 3 (isoform 1) has a predicted length of 473 residues and MW of 53 kDa. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetM-phase inducer phosphatase 3
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human;
Target Host SpeciesHuman
Immunogen DescriptionRecombinant human M-phase inducer phosphatase 3
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesDual specificity phosphatase Cdc25C; CDC25C; MPIP3;
Scientific BackgroundM-phase inducer phosphatase 3, also known as CDC25C, is a tyrosine phosphatase expressed predominantly in G2 phase. It directly dephosphorylates CDC2 and triggers entry into mitosis. M-phase inducer phosphatase 3 is also thought to suppress p53-induced growth arrest. At least 5 isoforms are produced by alternative splicing.