Pro-brain-derived neurotrophic factor (proBDNF), Mouse Monoclonal Antibody

Product Info

Product Name Pro-brain-derived neurotrophic factor (proBDNF), Mouse Monoclonal Antibody
Product Description google Mouse anti-Pro-brain-derived neurotrophic factor (proBDNF) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
Alternative Names Abrineurin
Application(s) FC, ICC
Antibody Host Mouse
Antibody Type Monoclonal
Specificity Human Not yet tested. Antibody may detect mouse and rat proBDNF protein due to high degree of amino acid sequence homology.
Species Reactivity Human, Mouse (Predicted), Rat
Immunogen Description A synthetic peptide (C-NGPKAGSRGLTS, aa: 47-58) of human proBDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proBDNF full-length protein.
Conjugate Unconjugated
Purity Description Protein G purified mouse IgG
Regulatory Status For research use only.

Specifications

Product Description Mouse anti-Pro-brain-derived neurotrophic factor (proBDNF) Monoclonal Antibody (Unconjugated), suitable for FC, ICC.
Application(s) FC, ICC
Application Details Flow Cytometry (2 ug/10⁶ cells).
Immunocytochemistry (1-2 µg/mL).
Other applications not yet tested. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Pro-brain-derived neurotrophic factor (proBDNF)
Specificity Human Not yet tested. Antibody may detect mouse and rat proBDNF protein due to high degree of amino acid sequence homology.
Target Host Species Human
Species Reactivity Human, Mouse (Predicted), Rat
Antibody Host Mouse
Antibody Type Monoclonal
Antibody Isotype IgG1, kappa
Clone Name BS375
Conjugate Unconjugated
Immunogen Description A synthetic peptide (C-NGPKAGSRGLTS, aa: 47-58) of human proBDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proBDNF full-length protein.
Purity Description Protein G purified mouse IgG
Format Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives.
Reconstitution Instructions Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.
Storage Instructions After reconstitution divide into aliquots and store at -20°C for a higher stability. Antibody contains no preservatives. Store at 2-8°C with an appropriate antibacterial agent. Use sterile methods. Highest purity Glycerol (1:1) may be added for an additional stability when stored at refrigerated or freezing temperatures. Avoid repetitive freeze/thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Abrineurin
Uniprot Number P23560
Uniprot Number/Name P23560 (BDNF_HUMAN)
Scientific Background Brain derived neurotrophic factor (BDNF) is synthesized as a precursor (proBDNF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proBDNF is synthesized in neurons and glia (eg., microglia), transported anterogradely and retrogradely and may be released in an activity dependent manner.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Detection of proBDNF expression in human neuroblastoma cell line SHSY-5Y by Flow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to proBDNF (cat # M-1729-100, 2 μg per ~10⁶ cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-200) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo Moxiflow^TM instrument and protocols.
proBDNF staining in fixed and permeabilized rat hippocampal neurons (7 DIV) by Immunocytochemistry. proBDNF-IR (red) is observed in neurites and cell bodies, consistent with expected cellular localization. Blue: Nuclei. Primary antibody concentration: 2 µg/mL; Magnification: 32X. Image courtesy of QBM Cell Science.

Citations & References