Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 4.0 µg/mL is recommended for WB. Human G1/S-specific cyclin-D1 has a predicted length of 295 residues and MW of 34 kDa. A concentration of 8.0 µg/mL is recommended to detect the protein in formalin fixed and paraffin embedded tissues as well as formalin/acetone fixed frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetG1/S-specific cyclin D1
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Human; mouse; rat;
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionRecombinant human G1/S-specific cyclin-D1 protein
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability.Avoid freeze-thaw cycles
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundG1/S-specific cyclin-D1 (CCND1) is a member of the Cyclin family which act as regulators of CDK kinases. CCND1 is essential for the control of the cell cycle at the G1/S (start) transition.