Product DescriptionMouse anti-Talin-1 Monoclonal Antibody (Unconjugated), suitable for WB.
Application DetailsWestern Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended to detect the protein in cells and tissues. This antibody reacts with both Talin-1 and Talin-2. It recognizes an epitope located on the intact Talin and a 190 kDa fragment. It does not recognize the 47 kDa fragment obtained by protease cleavage of chicken gizzard Talin. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
SpecificityThe specificity of this antibody has been confirmed by WB against the antigen. Human; mouse; rat; chicken; frog;
Target Host SpeciesChicken
Species ReactivityChicken, Frog, Human, Mouse, Rat
Immunogen DescriptionTalin purified from chicken gizzard
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTalin-1; TLN1; KIAA1027; TLN; Talin-2; TLN2; KIAA0320;
Scientific BackgroundTalin is a large, multidomain focal adhesion protein involved in the assembly of actin filaments. Talin interacts with the intracellular domains of integrins, vinculin and other focal adhesion proteins.