Application DetailsImmunohistochemistry (IHC) and Western Blotting (WB). A concentration of 2.0-4.0 µg/mL is recommended for WB. Rabbit TNNT3 (isoform TNT1) has a predicted length of 279 residues and a MW of 33 kDa. A concentration of 4.0-8.0 µg/mL is recommended to detect the protein in paraffin embedded tissues. Heat mediated antigen retrieval is required. A concentration of 4.0-8.0 µg/mL is recommended to detect the protein in frozen tissues. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTroponin T, fast skeletal muscle (TnTf)
SpecificityThe specificity of this antibody has been confirmed by WB and IHC against the antigen. Rat; rabbit; chicken;
Target Host SpeciesRabbit
Species ReactivityChicken, Rabbit, Rat
Immunogen DescriptionTroponin T from rabbit skeletal muscle
FormatLyophilized, Mouse ascites fluid, 1.2% sodium acetate, 2 mg BSA, with 0.01 mg NaN3 as preservative.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 1 mL of sterile-filtered PBS (pH 7.4) to achieve an antibody concentration of 100 µg/mL. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2 - 8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesTroponin T; TnTs; sTnT; TNNT1; TNNT2; TNNT3; TNT;
Scientific BackgroundTroponin is found on the thin filament of striated muscles and is composed of three subunits: troponin C, T and I. Troponin T binds tropomyosin. Three genes encode Tropomyosin T in a tissue specific manner; slow skeletal muscle (TNNT1), cardiac muscle (TNNT2) and fast skeletal muscle (TNNT3). All 3 forms of troponin T have multiple isoforms produced by alternative splicing.