Left: Analysis of UCHL1 expression in rat hippocampal section by Immunohistochemistry. UCHL1 protein was visualized with mouse antibody to UCHL1 (green, 1:5,000), and section co-stained with rabbit anti-FOX3/NeuN (R-3770-100, red, 1:2,000). Blue: DAPI nuclear stain. IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The UCHL1 antibody stains the cell body and dendrites of hippocampal neurons, while the FOX3 antibody labels nuclei of the neuronal cells. Right: Western blot analysis of UCHL1 expression in tissue lysates (green, 1:10,000).  protein standard,  rat brain,  rat spinal cord,  mouse brain,  mouse spinal cord,  pig brain,  pig spinal cord. The single band at 24 kDa corresponds to the UCHL1 protein.
Ubiquitin C Terminal Hydrolase 1 staining (red) in hippocampal cultures by Immunocytochemistry. Primary antibody dilution: 1:2,000. Blue: nuclear stain. Image courtesy of QBM Cell Science.