Confocal image of adult rat cerebellar cortex stained with M-1657-100 (green), our chicken polyclonal antibody to MAP2 (Catalog Number C-1382-50, red) and DNA (blue). The M-1657-100 antibody reveals synapses in the molecular layer (ML) strongly. Synaptic regions are also seen in the granule cell layer (GC). The perikarya of Purkinje cells (PC) are revealed with MAP2 antibody (4). Little staining is seen in the white matter (WM).
Left: VILIP-1 staining of rat cerebellum section by Immunohistochemistry. Section was stained with mouse antibody to VILIP-1 (red, 1:500) and rabbit antibody to GFAP (R-1374-50, green, 1:5,000). Blue: DAPI nuclear stain.IHC Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut to 45 um, and free-floating sections were stained. The VILIP-1 antibody reveals protein expressed in granule cell membranes and in synapses in the white matter and molecular layers of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astroglia. Right: Western blot analysis of VILIP-1 expression (green, 1:1,000) in tissue lysates.  protein standard,  rat brain  rat cerebellum, and  mouse brain. The band at around 20 kDa corresponds to VILIP-1 protein.
Co-localization of calretinin (green) and visinin (red) in retinal neuron visualized by Immunocytochemistry. Rat retinal primary cells (QBM cat# R-Ret-508) were cultured for 7 days. Image courtesy of QBM Cell Science.
Staining of calretinin (red) and VILIP1 (green) positive neurons by Immunocytochemistry in rat retina culture. Rat retinal primary cells (QBM cat# R-Ret-508) were cultured for 7 days. Image courtesy of QBM Cell Science.