Rat brain neural cultures stained with mouse anti-DCX (green), chicken polyclonal antibody to MAP2 (Catalog Number C-1382-50) (red) and DNA (blue). The mouse anti-DCX antibody (Catalog Number M-1648-100) reveals strong cytoplasmic staining in a population of small developing neurons. These cells are often found in small clumps as in these cultures, as in this example. Note that they are not positive for MAP2, which is characteristic of more mature neurons, and that the mature neuron shown is negative for DCX. This DCX antibody is therefore an excellent marker of developing neuronal cells.
Left: Analysis of doublecortin expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Cells were stained with mouse antibody to doublecortin (red, 1:1,000) and chicken anti-MAP2 antibody (C-1382-50, green, 1:10,000). Blue: DAPI nuclear stain. The doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons. Doublecortin antibody is an excellent marker of early developing neuronal cells. Right: Western blot analysis of doublecortin expression rat whole brain (green, 1:1,000).  protein standard,  adult brain,  embryonic E20 brain. Strong bands at 40 kDa and 45 kDa correspond to the doublecortin protein, detected only in the developing brain.