Product DescriptionMouse anti-Orexin receptor type 2 (Ox-2-R) Monoclonal Antibody (Unconjugated), suitable for WB, ELISA.
Application(s)ELISA, WB
Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetOrexin receptor type 2 (Ox-2-R)
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Species ReactivityHuman
Antibody HostMouse
Antibody TypeMonoclonal
Antibody IsotypeIgG
Clone Name1E3
ConjugateUnconjugated
Immunogen DescriptionPartial recombinant protein of human Orexin receptor type 2 (aa 1 to 55) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesOx2r; Hypocretin receptor type 2; HCRTR2
Scientific BackgroundOrexin receptor type 2 is a nonselective, high-affinity receptor for both orexin-A and orexin-B neuropeptides. It is involved in the central feedback mechanism that regulates feeding behaviour. It is a transmembrane protein and belongs to the G-protein coupled receptor 1 family.
WB detection of the antigen using mouse monoclonal antibody to human orexin receptor type 2, catalogue number M-840-100. Note the GST tag alone has a molecular weight of 26KDa.