Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetG-protein coupled receptor 24
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant protein of human G-protein coupled receptor 24 (aa 14 to 114) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Scientific BackgroundG-protein coupled receptor 24 belongs to the G-protein coupled receptor 1 family and binds melanin-concentrating hormone. It is coupled to both G proteins that inhibit adenylyl cyclase and G proteins that activate phosphoinositide hydrolysis. It is a transmembrane protein that is highly expressed in the brain, in particularly in the frontal cortex and hypothalamus. It is also expressed in the liver and heart at lower levels.
Western blot detection of G-protein coupled receptor 24 in human promyelocytic leukemia lysate using Mouse monoclonal antibody to human G-protein coupled receptor 24, catalogue number M-839-100.
Sandwhich ELISA using mouse monoclonal antibody to human G-protein coupled receptor 24 [3D7], catalogue number M-839-100. This antibody has a detection limit of 0.03ng/mL for recombinant GST tagged G-protein coupled receptor 24 protein.