SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. This antibody cross reacts with mouse and rat protein kinase minibrain homolog proteins.
Target Host SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Immunogen DescriptionPartial recombinant protein of human protein kinase minibrain homolog (aa 674 to 763) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.2
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Protein kinase minibrain homolog phosphorylates serine, threonine and tyrosine residues in its sequence as well as in exogenous substrates. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. There are 5 named isoforms produced by alternative splicing. It is expressed ubiquitously with highest levels in skeletal muscle, testis, fetal lung and kidney, and in the developing central nervous system. It localises to the nucleus speckle. This protein is overexpressed 1.5-fold in fetal Down syndrome brain and considered a strong candidate for learning defects associated with Down syndrome. It belongs to the protein kinase superfamily, CMGC ser/Thr protein kinase family and MNB/DYRK subfamilly.