Application DetailsThis antibody is recommended for WB, and sandwich ELISA. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetTranscription factor HES-1
SpecificitySpecificity has been confirmed by WB and direct ELISA against the antigen. Human. Other species have not been tested.
Target Host SpeciesHuman
Immunogen DescriptionPartial recombinant protein of human Transcription factor HES-1 (aa 36 to 143) with a GST tag.
Purity DescriptionProtein G purified immunoglobulin
FormatLyophilized from PBS pH 7.3
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAfter reconstitution keep aliquots at -20°C for higher stability or at 2-8°C with an appropriate antibacterial agent. Glycerol (1:1) may be added for additional stability. Avoid repetitive freeze/thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesHairy and enhancer of split 1; Hairy-like; HHL; Hairy homolog; HES1; HL; HRY
Transcription factor HES-1 is a transcriptional repressor of genes that require a basic helix-loop-helix protein for their transcription. It needs to form a complex with a corepressor protein (Groucho) to repress transcription. Transcription factor HES-1 has a particular type of basic domain which contains a helix-interrupting proline, that binds to the N-box (CACNAG) rather than the canonical E-box (CANNTG). It also consists of a C-terminal WRPW motif that is necessary for the interaction with Groucho. It localises to the nucleus.
Western blot analysis on human acute monocytic leukemia cell lysate using mouse monoclonal antibody to human Transcription factor HES-1, catalogue number M-841-100. This antibody detects a band at ~30KDa.