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Multi-Neurotrophin Screening, Human, Rapid™ ELISA assay
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Catalog Number
BEK-2227
- Product Name Multi-Neurotrophin Screening, Human, Rapid™ ELISA assay
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Product Description
The biosensis Multi-Neurotrophin RapidTM Screening ELISA kit has been designed to allow rapid screening and quantification of human NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum, plasma (EDTA and citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human serum and plasma samples.
This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin RapidTM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded.
NOTE: For research use only. Not for diagnostic and clinical purposes. - Alternative Names NGF; BDNF; NT3; NT4/5;
- Application(s) ELISA
- Specificity This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the mouse and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
- Species Reactivity Human
- Immunogen Description As for individual ELISA kits
- Range NGF (3.9 - 250 pg/mL) BDNF (7.8 - 500 pg/mL) NT3 (15.6 - 1,000 pg/mL) NT4/5 (15.6 - 1,000 pg/mL)
- Sensitivity Typical LODs achieved based on 150% of background OD:NGF: 3 pg/mLBDNF: 1 pg/mLNT3: 4 pg/mLNT4/5: 30 pg/mL
- Regulatory Status For research use only.
Product Info
-
Product Description
The biosensis Multi-Neurotrophin RapidTM Screening ELISA kit has been designed to allow rapid screening and quantification of human NGF, BDNF, NT3 and NT4/5 in cell culture supernatants, lysates, serum, plasma (EDTA and citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular human serum and plasma samples.
This two-plate kit consists of four sets of 6 strips for each Neurotrophin, allowing for the assay of 16 samples per Neurotrophin tested and a full range of standards, all run in duplicate. It provides the identical sensitivities and ranges that are achieved in the complete, individual ELISA kits, thus allowing easy progression into the larger individual assay sizes when needed. The Multi-Neurotrophin RapidTM Screening ELISA kit therefore presents a cost effective way to quickly screen multiple samples, which can then be published or used prior to a more extensive analysis with the individual kits. The kit comes complete with all detection reagents and is ready to use. Individual coated strips are provided and each set of standards and detection antibodies are color-coded.
NOTE: For research use only. Not for diagnostic and clinical purposes. -
Related Products
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Neurotrophin-4/5 (NT-4/5), Human, Mouse, Rat, Monkey, Rapid™ ELISA assay
Neurotrophin-3 (NT-3), Human, Mouse, Rat, Rapid™ ELISA assay
- Application(s) ELISA
- Application Details ELISA. For the quantification of Multi-Neurotrophin Screening in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
- Target Multi-Neurotrophin Screening
- Specificity This Multi-Neurotrophin Screening kit uses the same antibodies as in the individual Rapid ELISA kits. Each kit has been tested for cross-reactivity with other closely related neurotrophins and no cross-reactivity has been observed at 25 ng/mL of each neurotrophin tested. The antibodies used in this kit detect the mouse and rat form of each neurotrophin, but with varying efficiency depending on the target protein.
- Target Host Species Human
- Species Reactivity Human
- Immunogen Description As for individual ELISA kits
- Range NGF (3.9 - 250 pg/mL) BDNF (7.8 - 500 pg/mL) NT3 (15.6 - 1,000 pg/mL) NT4/5 (15.6 - 1,000 pg/mL)
- Sensitivity Typical LODs achieved based on 150% of background OD:NGF: 3 pg/mLBDNF: 1 pg/mLNT3: 4 pg/mLNT4/5: 30 pg/mL
- Sample Type Cell Lysates, Culture Supernatant, Tissue Homogenates
- Detection Method Colorimetric
- Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s) (6 strips/48 wells per neurotrophin target), protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
- Storage Instructions Store at 2-8°C
- Storage Temperature 2-8°C
- Batch Number Please see item label.
- Expiration Date 12 months from purchase.
- Alternative Names NGF; BDNF; NT3; NT4/5;
- Uniprot Number P23560; P01138; P20783; P34130
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Uniprot Number/Name
P23560 (BDNF_HUMAN)
P01138 (NGF_HUMAN)
P20783 (NTF3_HUMAN)
P34130 (NTF4_HUMAN) - Scientific Background The Neurotrophin family of growth factors in all mammals including human has four members including Nerve Growth Factor (NGF), Brain-Derived Neurotrophic Factor (BDNF), Neurotrophin 3 (NT3) and Neurotrophin 4/5 (NT4/5). These are all essential to brain development, maturation and adult function, particularly for cell differentiation, survival, death and synaptic regulation.
- Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
- Shipping Temperature 2-8°C (on cold packs)
- UNSPSC CODE 41116158
- Regulatory Status For research use only.
Specifications
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Specific References
Lamb WDB (2021) "Potential of Muller glia-derived extracellular vesicles for retinal neuroprotection." PhD Thesis Application: Human cell culture supernatant.
Elisa A et al. (2021) "Aging is associated with cardiac autonomic nerve fiber depletion and reduced cardiac and circulating BDNF levels." J Geriatr Cardiol. 18(7): 549–559 Application: Rat serum.
Woo JH et al. (2019) "Effect of Dehydrocostus Lactone Isolated from the Roots of Aucklandia lappa on the Apoptosis of Endometriotic Cells and the Alternative Activation of Endometriosis-Associated Macrophages." Am J Chin Med. [Epub ahead of print] Application: Human cell culture supernatants.
Kalinowska-Lyszczarz A et al. (2018) "Immune-cell BDNF expression in treatment-naive relapsing-remitting multiple sclerosis patients and following one year of immunomodulation therapy." Neurol Neurochir Pol. [In Press] Application: Human PBMC cell lysates.
Lindsay SL et al. (2016) "Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells." Stem Cell Reports. 6(5):729-42 Application: MSC-conditioned medium/cell supernatant.
Carnevale G et al. (2016) "Human dental pulp stem cells expressing STRO-1, c-kit and CD34 markers in peripheral nerve regeneration." J Tissue Eng Regen Med. [Epub ahead of print] Application: Supernatant of human dental pulp stem cells (hDPSCs).