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Myelin basic protein (MBP), Chicken Polyclonal Antibody

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Catalog Number

C-1383

Product Info

Product Name Myelin basic protein (MBP), Chicken Polyclonal Antibody
Product Description google Chicken anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Alternative Names Myelin Basic Protein; Myelin A1 protein; Myelin membrane encephalitogenic protein;
Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
Antibody Host Chicken
Antibody Type Polyclonal
Specificity The specificity of this antibody has been confirmed by WB. This antibody stains a prominent band at approx. 20 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Hu, Rat, Ms, Bov. Predicted to react with other mammalian tissues due to sequence homology.
Species Reactivity Bovine, Human, Mouse, Other Mammals (Predicted), Rat
Immunogen Description Three peptide sequences conserved in higher verterbrate MBP protein.
Conjugate Unconjugated
Purity Description Affinity purified
Regulatory Status For research use only.

Specifications

Product Description Chicken anti-Myelin basic protein (MBP) Polyclonal Antibody (Unconjugated), suitable for WB, IHC-Frozen, IHC-Paraffin-embedded, ICC.
Application(s) ICC, IHC-Frozen, IHC-Paraffin-embedded, WB
Application Details Western Blotting (WB), Immunocytochemistry (ICC) and Immunohistochemistry (IHC). The recommended dilution for WB is 1:5,000-10,0000 and 1:500-1,000 for IC and IH. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target Myelin basic protein (MBP)
Specificity The specificity of this antibody has been confirmed by WB. This antibody stains a prominent band at approx. 20 kDa. A suitable control tissue is rat spinal cord or peripheral nerve homogenate. The major isoforms of MBP run as a closely spaced double of 22 kDa and 18 kDa. Hu, Rat, Ms, Bov. Predicted to react with other mammalian tissues due to sequence homology.
Target Host Species Human
Species Reactivity Bovine, Human, Mouse, Other Mammals (Predicted), Rat
Antibody Host Chicken
Antibody Type Polyclonal
Antibody Isotype IgY
Conjugate Unconjugated
Immunogen Description Three peptide sequences conserved in higher verterbrate MBP protein.
Purity Description Affinity purified
Format Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, without preservatives
Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage Instructions After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Myelin Basic Protein; Myelin A1 protein; Myelin membrane encephalitogenic protein;
Uniprot Number P02686
Uniprot Number/Name P02686 (MBP_HUMAN)
Scientific Background Myelin is a membrane characteristic of the nervous tissue and functions as an insulator to increase the velocity of the stimuli being transmitted between a nerve cell body and its target. Myelin isolated from human and bovine nervous tissue is composed of approximately 80% lipid and 20% protein, and 30% of the protein fraction constitutes myelin basic protein (MBP). MBP is an 'intrinsically unstructured' protein with a high proportion (approximately 75%) of random coil, but postulated to have core elements of beta-sheet and alpha-helix. MBP is a major protein in CNS myelin and is expressed specifically in the nervous system. A detailed immunochemical examination of monoclonal and polyclonal antibody responses to MBP and its peptides has revealed the existence of as many as 27 antigenic determinants, many of them conformational. Topological mapping of the potential antigenic determinants onto a model of MBP secondary structure places these determinants within 11 separate regions of the molecule, including those portions that have been found to be encephalitogenic. The message for myelin basic protein is selectively translocated to the ends of the cell processes. Immunization with myelin-associated antigens including MBP significantly promotes recovery after spinal cord contusion injury in the rat model. FUNCTION: Is, with PLP, the most abundant protein component of the myelin membrane in the CNS. Has a role in both the formation and stabilization of this compact multilayer arrangement of bilayers. Each splice variant and charge isomer may have a specialized function in the assembly of an optimized, biochemically functional myelin membrane (By similarity). SUBUNIT: Homodimer (By similarity). SUBCELLULAR LOCATION: Myelin membrane; peripheral membrane protein; cytoplasmic side. Cytoplasmic side of myelin. TISSUE SPECIFICITY: Found in both the central and the peripheral nervous system.
Shipping Temperature 25°C (ambient)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

Image shows rat mixed neuron/glial cultures stained with Chicken polyclonal antibody to Myelin Basic Protein antibody C-1383-50 (green). Blue is a DNA stain. Note that the antibody stains an oligodendrocyte and some membrane shed from this cell. Other cells in the field include neurons, astrocytes, microglia and fibroblasts, all of which are completely negative.
Left: Analysis of MBP expression in rat brain cerebellum by Immunohistochemistry with chicken anti-MBP antibody (1:5,000, green). Section was co-stained with rabbit antibody to NF-L (R-1392-50, 1:5,000, red). The MBP antibody stains oligodendrocytes and myelin sheathes around axons, while the NF-L antibody labels dendrites and axons of neuronal cells. Right: Western blot analysis of tissue lysates with chicken antibody to MBP (1:10,000). [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands between 14-22 kDa correspond to various alternate transcripts of the single MBP gene.

Citations & References

Specific References Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.