NACHT, LRR and PYD domains-containing protein 3 (NLRP3), Goat Polyclonal Antibody

Product Info

Product Name NACHT, LRR and PYD domains-containing protein 3 (NLRP3), Goat Polyclonal Antibody
Product Description google Goat anti-NACHT, LRR and PYD domains-containing protein 3 (NLRP3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Alternative Names NLRP3; CIAS1; FCU; MWS; FCAS; NALP3; C1orf7; PYPAF1; AII/AVP; AGTAVPRL; CRYOPYRIN; cold autoinflammatory syndrome 1; cryopyrin; Muckle-Wells syndrome; chromosome 1 open reading frame 7; PYRIN-containing APAF1-like protein 1; neuronal apoptosis inhibitor protein 3; angiotensin/vasopressin receptor AII/AVP-like; NLR family, pyrin domain containing 3; AII; AVP; CLR1.1; FLJ95925; AII/AVP receptor-like; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; PYRIN-containing APAF1-like protein 1; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Application(s) FC, ICC, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with CIAS1 / cryopyrin ( C Terminus) from Human. Variants (NP_001073289.1; NP_004886.3) encode the same isoform.
Species Reactivity Bovine (Predicted), Human, Rat (Predicted)
Immunogen Description A synthetic peptide consisting of amino acids, C-EKPELTVVFEPSW
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-NACHT, LRR and PYD domains-containing protein 3 (NLRP3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, ICC, FC.
Application(s) FC, ICC, Pep-ELISA
Application Details Peptide ELISA (1:28000), Immunofluorescence (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target NACHT, LRR and PYD domains-containing protein 3 (NLRP3)
Specificity Reacts with CIAS1 / cryopyrin ( C Terminus) from Human. Variants (NP_001073289.1; NP_004886.3) encode the same isoform.
Target Host Species Human
Species Reactivity Bovine (Predicted), Human, Rat (Predicted)
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-EKPELTVVFEPSW
Sequence EKPELTVVFEPSW
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names NLRP3; CIAS1; FCU; MWS; FCAS; NALP3; C1orf7; PYPAF1; AII/AVP; AGTAVPRL; CRYOPYRIN; cold autoinflammatory syndrome 1; cryopyrin; Muckle-Wells syndrome; chromosome 1 open reading frame 7; PYRIN-containing APAF1-like protein 1; neuronal apoptosis inhibitor protein 3; angiotensin/vasopressin receptor AII/AVP-like; NLR family, pyrin domain containing 3; AII; AVP; CLR1.1; FLJ95925; AII/AVP receptor-like; NACHT domain-, leucine-rich repeat-, and PYD-containing protein 3; NACHT, LRR and PYD containing protein 3; PYRIN-containing APAF1-like protein 1; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 3
Uniprot Number Q96P20
Uniprot Number/Name Q96P20 (NLRP3_HUMAN)
Scientific Background NLRP (also known as CIAS1), is a gene that encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif, that interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis (Ref. NCBI).
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Immunofluorescence analysis: paraformaldehyde fixed U937 cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (4 µg/mL). B. Flow cytometry analysis: paraformaldehyde fixed human peripheral blood monocytes (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody. NB: Gated on Granulocytes.

Citations & References