NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3), Goat Polyclonal Antibody

Product Info

Product Name NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3), Goat Polyclonal Antibody
Product Description google Goat anti-NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Alternative Names Sirt3; sirtuin 3; sirtuin 3 (silent mating type information regulation 2; homolog) 3; 2310003L23Rik; AI848213; Sir2l3; NAD-dependent deacetylase sirtuin-3; OTTMUSP00000027441; OTTMUSP00000027442; OTTMUSP00000027444; OTTMUSP00000027445; SIR2-like protein 3; SIRT3L mitochondrial; mSIR2L3; mitochondrial protein lysine deacetylase; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3
Application(s) FC, ICC, WB, Pep-ELISA
Antibody Host Goat
Antibody Type Polyclonal
Specificity Reacts with Sirtuin 3 (mouse) from Mouse. This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1.
Species Reactivity Human, Mouse, Pig, Rat
Immunogen Description A synthetic peptide consisting of amino acids, C-RASGIPASKLVEAH
Conjugate Unconjugated
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Regulatory Status For research use only.

Specifications

Product Description Goat anti-NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3) Polyclonal Antibody (Unconjugated), suitable for Pep-ELISA, WB, ICC, FC.
Application(s) FC, ICC, WB, Pep-ELISA
Application Details Peptide ELISA (1:164000), Western Blot (1-3 µg/mL), Immunocytochemistry (10 µg/mL), Flow Cytometry (10 µg/mL). Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
Target NAD-dependent protein deacetylase sirtuin-3, mitochondrial (SIRT3)
Specificity Reacts with Sirtuin 3 (mouse) from Mouse. This antibody is expected to recognize both reported isoforms (NP_071878.2; NP_001171275.1). Reported variants represent identical proteins: NP_071878.2; NP_001120823.1.
Target Host Species Mouse
Species Reactivity Human, Mouse, Pig, Rat
Antibody Host Goat
Antibody Type Polyclonal
Antibody Isotype IgG
Conjugate Unconjugated
Immunogen Description A synthetic peptide consisting of amino acids, C-RASGIPASKLVEAH
Sequence RASGIPASKLVEAH
Purity Description Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Format Liquid antibody. Supplied at 0.5 mg/mL in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Storage Instructions Upon receipt, aliquot and store at -20°C long-term. Store at 2-8°C short-term (up to 2 weeks). Minimize freezing and thawing.
Batch Number Please see item label.
Expiration Date 12 months after date of receipt (unopened vial).
Alternative Names Sirt3; sirtuin 3; sirtuin 3 (silent mating type information regulation 2; homolog) 3; 2310003L23Rik; AI848213; Sir2l3; NAD-dependent deacetylase sirtuin-3; OTTMUSP00000027441; OTTMUSP00000027442; OTTMUSP00000027444; OTTMUSP00000027445; SIR2-like protein 3; SIRT3L mitochondrial; mSIR2L3; mitochondrial protein lysine deacetylase; silent mating type information regulation 2, (S.cerevisiae, homolog)-like 3
Uniprot Number Q9NTG7
Uniprot Number/Name Q9NTG7 (SIR3_HUMAN)
Scientific Background NAD-dependent protein deacetylas. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism (PubMed:24252090). Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116161
Regulatory Status For research use only.

Images, Protocols & SDS

A. Western Blot: (0.3 µg/mL) staining of Mouse Heart lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence. B. Immunofluorescence analysis: of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL). C. Flow cytometry analysis: of paraformaldehyde fixed NIH3T3 cells (blue line), permeabilized with 0.5% Triton, showing a low level of staining. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Citations & References