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Nerve growth factor (2.5S), Mouse, Purified Native Protein
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Catalog Number
PE-019
- Product Name Nerve growth factor (2.5S), Mouse, Purified Native Protein
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Product Description
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
- Alternative Names mouse NGF; beta NGF
- Application(s) Cell Culture, ELISA, WB
- Purity Description Greater than 90% (as determined by SDS electrophoresis)
- Purity % > 90%
- Regulatory Status For research use only.
Product Info
-
Product Description
Mouse NGF (2.5S) was isolated from mouse submaxillary glands by method of Mobley et al (1976) and is a form of beta-NGF that has identical biological properties. NGF is known to regulate the survival and development of certain sympathetic and sensory neurons. It is a dimer with 2 identical polypeptide chains and dimeric molecular weight of approximately 26,500 Da. Isolation and purification of NGF from mouse submaxillary glands yields preparations of NGF (2.5S) with identical biological activity but with cleavages at the amino terminus (with the loss of 8 amino acids) and/or at the carboxy-terminus (with the loss of arginine). These preparations are named 2.5 NGF (see reference below).
- Application(s) Cell Culture, ELISA, WB
- Application Details Stimulates neurite outgrowth in rat PC12 cells
- Target Nerve growth factor, beta (Beta-NGF)
- Target Host Species Mouse
- Antigen Produced In Native
- Biological Activity Stimulates neurite outgrowth in rat PC12 cells
- Purity Description Greater than 90% (as determined by SDS electrophoresis)
- Purity % > 90%
- Format Lyophilized from PBS, pH 7.4 without preservatives.
- Reconstitution Instructions Reconstitute with sterile-filtered, ultrapure water to a concentration of 1 mg/mL.
- Storage Instructions Store lyophilized protein at 2-8°C. After reconstitution, store at 2-8°C short term. Store long-term at -20°C to -80°C. Avoid repeated freezing and thawing. See expiration date for shelf-life estimates, actual times may vary depending upon experimental conditions and laboratory handling.
- Batch Number Please see item label.
- Expiration Date 12 months after date of receipt for lyophilized material. Reconstituted material, 5 days at 4C, 30 days -20°C, 60 days -70°C for highest activity Avoid repeated freezing and thawing. Use insulated storage containers for best results.
- Alternative Names mouse NGF; beta NGF
- Uniprot Number P01139
- Uniprot Number/Name P01139 (NGF_MOUSE)
- Scientific Background Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
- Shipping Temperature 25°C (ambient)
- UNSPSC CODE 12352202
- Regulatory Status For research use only.
Specifications
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Specific References
Wang YN et al (2021) Slit3 secreted from M2-like macrophages increases sympathetic activity and thermogenesis in adipose tissue. Nat Metab. 3(11):1536-1551.
Walters RR et al (2021) Pharmacokinetics and Immunogenicity of Frunevetmab in Osteoarthritic Cats Following Intravenous and Subcutaneous Administration. Front Vet Sci. 8:687448.
Carter DR et al (2016) Glutathione biosynthesis is upregulated at the initiation of MYCN-driven neuroblastoma tumorigenesis. Mol Oncol. 2016 Jun;10(6):866-78.
Goodhill GJ et al (2015) The dynamics of growth cone morphology. BMC Biol. 2015 Feb;13(10).
Coulson EJ et al (2015) Neurotrophin-tyrosine kinase receptor signaling. United States Patent Application 20150344535.
Gearing, DP et al (2013) A fully caninised anti-NGF monoclonal antibody for pain relief in dogs. BMC Vet Res. 9:226.
Matusica D et al (2013) An intracellular domain fragment of the p75 neurotrophin receptor (p75(NTR)) enhances tropomyosin receptor kinase A (TrkA) receptor function. J Biol Chem. 2013 Apr 19;288(16):11144-54.
Yuan J et al (2013) Optimality and saturation in axonal chemotaxis. Neural Comput. 2013 Apr;25(4):833-53.
Sykes AM et al (2012) The effects of transmembrane sequence and dimerization on cleavage of the p75 neurotrophin receptor by gamma-secretase. J Biol Chem. 2012 Dec 21;287(52):43810-24.
E.M. Forbes et al (2012) Calcium and cAMP Levels Interact to Determine Attraction versus Repulsion in Axon Guidance. Neuron. 2012 May 10;74(3):490-503.
- General References Mobley WC, Schenker A, Shooter EM (1976) Biochemistry. 15(25) pp. 5543-52.