Nerve growth factor, pro- (proNGF), Human, Rapid™ ELISA assay

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Catalog Number
BEK-2226

    Product Info

  • Product Name Nerve growth factor, pro- (proNGF), Human, Rapid™ ELISA assay
  • Product Description google The Biosensis proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

    This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance.

    This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF!

    Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately.

    This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names pro-beta nerve growth factor; proNGF; NGF
  • Application(s) ELISA
  • Specificity Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
  • Species Reactivity Human
  • Immunogen Description Recombinant human, wild-type proNGF protein, made in E.coli
  • Range 0.078 - 5 ng/mL
  • Sensitivity Typical limit of detection (LOD) for proNGF is <60 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
  • Regulatory Status For research use only.

    Specifications

  • Product Description The Biosensis proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.

    This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance.

    This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF!

    Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately.

    This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Related Products Nerve growth factor (Beta-NGF), Human, Rapid™ ELISA assay
    Mature NGF/proNGF Combo (BEK-2212/2226), Human, Rapid™ ELISA assay
    Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Serum, Plasma (Heparin), Culture Supernatant, Cell Lysates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Nerve growth factor, pro- (proNGF)
  • Specificity Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
  • Target Cross-Reactivity (ELISA) Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description Recombinant human, wild-type proNGF protein, made in E.coli
  • Range 0.078 - 5 ng/mL
  • Sensitivity Typical limit of detection (LOD) for proNGF is <60 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
  • Sample Type Cell Lysates, Culture Supernatant, Plasma (Heparin), Serum
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C.
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date 12 months from purchase.
  • Alternative Names pro-beta nerve growth factor; proNGF; NGF
  • Uniprot Number P01138
  • Uniprot Number/Name P01138 (NGF_HUMAN)
  • Scientific Background Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References March B et al. (2021). ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine.

    Mossa AH et al. (2020). Imbalance of nerve growth factor metabolism in aging women with overactive bladder syndrome. World J Urol. [Epub ahead of print]. Application: Human urine.

    Rowe CW et al. (2019). The precursor for nerve growth factor (proNGF) is not a serum or biopsy-rinse biomarker for thyroid cancer diagnosis. BMC Endocr Disord. 19(1):128. Application: Human serum.

    Stapledon CJM et al. (2019). Human osteocyte expression of Nerve Growth Factor: The effect of Pentosan Polysulphate Sodium (PPS) and implications for pain associated with knee osteoarthritis. PLoS One. 14(9):e0222602. Application: Human primary culture supernatant.

    Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Human urine.

    Sherif IO & Al-Gayyar MMH (2018). Oleuropein potentiates anti-tumor activity of cisplatin against HepG2 through affecting proNGF/NGF balance. Life Sci. [Epub ahead of print]. Application: Human cell culture.