The Biosensis Mouse and Rat proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards.
This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'.
This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide.
This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Alternative Namespro-beta nerve growth factor; proNGF; NGF
Application(s)ELISA
SpecificityMouse proNGF. Expected to detect rat proNGF due to high degree of sequence homology. Detects human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF.
Species ReactivityMouse, Rat
Immunogen DescriptionRecombinant mouse, wild-type proNGF protein, made in E.coli
Range0.156 - 10 ng/mL
SensitivityTypical limit of detection (LOD) for mouse proNGF is less than 50 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
The Biosensis Mouse and Rat proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rodent full-length proNGF protein in less than 4 hours in cell culture supernatants and cell lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application.
This ELISA kit contains a recombinant mouse proNGF standard expressed in E.coli and consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards.
This ELISA kit is expected to detect rat proNGF due to high degree of homology (96%) with mouse proNGF based on amino acid sequence, and the ability of this kit in detecting proNGF in rat PC12 cell lysates. In the absence of a true rat proNGF standard, results may be expressed as 'mouse proNGF equivalents'.
This ELISA kit shows only 20% reactivity with the human form of proNGF and is therefore not suitable to quantify human proNGF. No cross-reactivity was observed with mature mouse NGF and full-length proBDNF when tested in assay buffer. The antibodies used in this ELISA kit bind epitopes within the pro-domain (capture) and mature domain (detection) of the protein, thus this ELISA assay does not detect the pro-domain peptide.
This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Application DetailsELISA. For the quantification of Nerve growth factor, pro- (proNGF) in Culture Supernatant, Cell Lysates, Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
TargetNerve growth factor, pro- (proNGF)
SpecificityMouse proNGF. Expected to detect rat proNGF due to high degree of sequence homology. Detects human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF.
Target Cross-Reactivity (ELISA)Reacts with human proNGF (about 20% reactivity). Does not cross-react with proBDNF and mature NGF. Mature NGF spiked into brain homogenate does not interfere with proNGF quantification.
Target Host SpeciesMouse
Species ReactivityMouse, Rat
Immunogen DescriptionRecombinant mouse, wild-type proNGF protein, made in E.coli
Range0.156 - 10 ng/mL
SensitivityTypical limit of detection (LOD) for mouse proNGF is less than 50 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
Scientific BackgroundNerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released.
Shipping StatementFor order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
Sirmakesyan S et al. (2023) "Synthesis and secretion of Nerve Growth Factor is regulated by Nitric Oxide in bladder cells in vitro under a hyperglycemic environment" Nitric Oxide. [Epub ahead of print] Application: Rat cell lysates.
Pathare-Ingawale P et al. (2023) Association between proNGF receptors and apoptotic factors in human placentae Placenta. [Epub ahead of print]. Application: Human placental tissue lysates.
Luu BE et al. (2022) Modulation of diabetic kidney disease markers by an antagonist of p75NTR in streptozotocin-treated mice Gene. [Epub ahead of print]. Application: Mouse kidney RIPA homogenates.
Mossa A et al. (2021). Adaptation to partial urethral obstruction in healthy aging LOU rats and the role of nerve growth factor signaling pathway in the bladder. Exp Gerontol. [Epub ahead of print]. Application: Rat urine.
Sugimoto J et al. (2021). Fabry disease-associated globotriaosylceramide induces mechanical allodynia via activation of signaling through proNGF p75NTR but not mature NGF TrkA. Eur. J. Pharmacol. 895. Application: Mouse tissue homogenate (RIPA).
Mossa AH et al. (2020). Antagonism of proNGF or its receptor p75NTR reverses remodelling and improves bladder function in a mouse model of diabetic voiding dysfunction. Diabetologia. [Epub ahead of print]. Application: Mouse bladder extracts (RIPA).
Ryu JC et al. (2018). Role of proNGF/p75 signaling in bladder dysfunction after spinal cord injury. J Clin Invest. [Epub ahead of print]. Application: Mouse urine.