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Nerve growth factor receptor, extracellular domain (NGFR/p75ECD), Human, Rapid™ ELISA assay

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Catalog Number

BEK-2239

Product Info

Product Name Nerve growth factor receptor, extracellular domain (NGFR/p75ECD), Human, Rapid™ ELISA assay
Product Description google
The Biosensis NGFR/p75^ECD Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit s a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application.

This ELISA kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a biotinylated mouse monoclonal anti-p75^ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards. A human p75^ECD positive control (QC sample) is provided to assure consistent assay performance.

This NGFR/p75^ECD ELISA kit employs a recombinant human p75^ECD-Fc chimera. While there is a current lack of a commercially available, true human p75^ECD standard, this ELISA kit allows quantification of human p75^ECD as p75^ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75^ECD.

Note: For research use only. Not for diagnostic and clinical purposes.
Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16; NGFR/p75ECD; p75ECD;
Application(s) ELISA
Specificity Human. This ELISA kit does not cross-react with mouse p75^ECD protein.
Species Reactivity Human
Immunogen Description Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Range 62.5 - 4,000 pg/mL
Sensitivity < 50 pg/mL
Regulatory Status For research use only.

Specifications

Product Description
The Biosensis NGFR/p75^ECD Rapid^TM enzyme-linked immunosorbent assay (ELISA) Kit s a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine only if used as directed. Please refer to the kit protocol for specific use instructions for urine application.

This ELISA kit consists of a pre-coated mouse monoclonal anti-p75^ECD capture antibody, a biotinylated mouse monoclonal anti-p75^ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75^ECD present in samples and protein standards. A human p75^ECD positive control (QC sample) is provided to assure consistent assay performance.

This NGFR/p75^ECD ELISA kit employs a recombinant human p75^ECD-Fc chimera. While there is a current lack of a commercially available, true human p75^ECD standard, this ELISA kit allows quantification of human p75^ECD as p75^ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75^ECD.

Note: For research use only. Not for diagnostic and clinical purposes.
Related Products Nerve growth factor receptor, extracellular domain (NGFR/p75ECD), Mouse, Rapid™ ELISA assay
Application(s) ELISA
Application Details ELISA. For the quantification of Nerve growth factor receptor, extracellular domain (NGFR/p75ECD) in Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
Target Nerve growth factor receptor, extracellular domain (NGFR/p75ECD)
Specificity Human. This ELISA kit does not cross-react with mouse p75^ECD protein.
Target Cross-Reactivity (ELISA) This ELISA kit does not cross-react with mouse p75ECD protein.
Target Host Species Human
Species Reactivity Human
Immunogen Description Recombinant human p75 (AA 1-250) -Fc chimera, made in 293 cells
Range 62.5 - 4,000 pg/mL
Sensitivity < 50 pg/mL
Sample Type Urine
Detection Method Colorimetric
Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers, substrate buffer and detailed protocols.
Storage Instructions Store at 2-8°C.
Storage Temperature 2-8°C
Batch Number Please see item label.
Expiration Date 12 months from purchase.
Alternative Names Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16; NGFR/p75ECD; p75ECD;
Uniprot Number P08138
Uniprot Number/Name P08138 (TNR16_HUMAN)
Scientific Background The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls.
Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
Shipping Temperature 2-8°C (on cold packs)
UNSPSC CODE 41116158
Regulatory Status For research use only.

Images, Protocols & SDS

Parallelism of six serially diluted human urine samples from healthy donors.
This standard curve generated in our laboratories is for demonstration purposes only, but can be used as a guide to expected performance. A standard curve should be generated for each assay.
Protocols & Application Notes Technical Note #1 - ELISA Assay Validation

Technical Note #2 - ELISA Data Analysis

Calculator #1 - Quantification of Antigen Amount in Tissues and Lysates by ELISA
Kit Protocol

Assay Diluent A and Assay Diluent E are identical products.

This name change is for internal documentation purposes only, and assay performance is unaffected.

For kits supplied with Assay Diluent A:
BEK-2239_as_at_May2023_Assay Diluent A.pdf

For kits supplied with Assay Diluent E:
BEK-2239_as_at_May2023_Assay Diluent E.pdf
SDS Link
MSDS_Rapid_ELISA_as_at_Oct2023.pdf

Citations & References

Specific References
Covarrubias C et al. (2023) Decrease in the Ratio proBDNF/BDNF in the Urine of Aging Female Patients with OAB Metabolites. 13(6), 723. Application: Human, Urine.

Yamada S et al. (2021) Ratio of urinary N-terminal titin fragment to urinary creatinine is a novel biomarker for amyotrophic lateral sclerosis J Neurol Neurosurgeon Psychiatry 92(10):1072-1079 Application: Human Urine
General References The following references give a general background on the suitability of p75^ECD as a biomarker for ALS disease progression.

Shepheard et al. (2017) Neurology 2017 Feb 22. doi: 10.1212/WNL.0000000000003741. [Epub ahead of print].

Shepheard et al. (2014) PLoS ONE 9(1): e87398. doi:10.1371/journal.pone.0087398.

DiStefano & Johnson (1988) Proc Natl Acad Sci USA 85: 270-274.