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Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Chicken Polyclonal Antibody

As low as US$317.00
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Catalog Number
C-1386
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    Product Info

  • Product Name Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Polyclonal Antibody (Unconjugated), suitable for WB, ELISA and Immunostaining.
  • Alternative Names NF-H; NFH; NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845
  • Application(s) ELISA, IF, ICC, IHC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Species cross-reactivity includes human, rat, mouse, cow, pig, horse and dog. This antibody reacts with phosphorylated NF-H and is seen as a band at approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. Predicted to react with other mammalian tissues due to sequence homology.
  • Species Reactivity Bovine, Dog, Horse, Human, Mouse, Other Mammals (Predicted), Pig, Rat
  • Immunogen Description This antibody has been made against biochemically isolated NF-H purified from bovine spinal cord.
  • Conjugate Unconjugated
  • Purity Description IgY Preparation
  • Regulatory Status For research use only.

    Specifications

  • Product Description Chicken anti-Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Polyclonal Antibody (Unconjugated), suitable for WB, ELISA and Immunostaining.
  • Related Products Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Mouse Monoclonal Antibody (NAP4)
    Neurofilament heavy polypeptide, phosphorylated, (pNF-H), Rabbit Polyclonal Antibody
    Neurofilament Heavy, phosphorylated and non-phosphorylated (pNF-H), Rabbit Polyclonal Antibody
  • Application(s) ELISA, IF, ICC, IHC, WB
  • Application Details Western blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF), and Immunohistochemistry (IHC). A dilution of 1:20,000-50,000 is recommended for WB. A dilution of 1:20,000 is recommended for ICC/IF and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
  • Target Neurofilament heavy polypeptide, phosphorylated (pNF-H)
  • Specificity Species cross-reactivity includes human, rat, mouse, cow, pig, horse and dog. This antibody reacts with phosphorylated NF-H and is seen as a band at approx 200 kDa in WB. Refer to publication by Shaw et al (2005) for the use of this antibody in an ELISA to detect NF-H. Predicted to react with other mammalian tissues due to sequence homology.
  • Target Host Species Bovine
  • Species Reactivity Bovine, Dog, Horse, Human, Mouse, Other Mammals (Predicted), Pig, Rat
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Antibody Isotype IgY
  • Conjugate Unconjugated
  • Immunogen Description This antibody has been made against biochemically isolated NF-H purified from bovine spinal cord.
  • Purity Description IgY Preparation
  • Format Lyophilized from PBS buffer pH 7.2-7.6 with 5mM sodium azide.
  • Reconstitution Instructions Spin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
  • Storage Instructions Store lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
  • Batch Number Please see item label.
  • Expiration Date 12 months after date of receipt (unopened vial).
  • Alternative Names NF-H; NFH; NF-200; NF200; NF-H; NEFH; N52; Neurofilament heavy polypeptide; Neurofilament triplet H protein; 200 kDa neurofilament protein; KIAA0845
  • Uniprot Number P12036
  • Uniprot Number/Name P12036 (NFH_HUMAN)
  • Scientific Background Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)
  • Shipping Temperature 25°C (ambient)
  • UNSPSC CODE 41116161
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1386-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Chicken pAb, (red, 1:5,000) and co-stained with product R-1374-50, Glial Fibrillary Acidic Protein (GFAP), Rabbit pAb (green). The blue is DAPI staining of nuclear DNA. Method: following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free floating sections were stained. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes.

  • Analysis by western blot of pNF-H expression in spinal cord lysates with C-1386-50, (green, 1:20,000) with strong bands at approx 200-220kDa corresponding to the phosphorlylated form of NF-H. Lane 1: MWM; Lane 2: rat; Lane 3: mouse; Lane 4: cow. The protein from different species is known to have different SDS-PAGE molecular weights, with large species generally expressing larger proteins. Smaller proteolytic fragments of NF-H are also detected in spinal cord preparations with this antibody. The antibody does not recognize non-phosphorylated forms of NF-H.