Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1386-50, Neurofilament heavy polypeptide, phosphorylated (pNF-H), Chicken pAb, (red, 1:5,000) and co-stained with product R-1374-50, Glial Fibrillary Acidic Protein (GFAP), Rabbit pAb (green). The blue is DAPI staining of nuclear DNA. Method: following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 uM, and free floating sections were stained. The NF-H antibody labels network of axons of different neurons, while the GFAP antibody stains astrocytes.