Neurofilament light polypeptide (NF-L), Chicken Polyclonal Antibody

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    Product Info

  • Product Name Neurofilament light polypeptide (NF-L), Chicken Polyclonal Antibody
  • Product Description google Chicken anti-Neurofilament light polypeptide (NF-L), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
  • Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; NFL; neurofilament light chain; Neurofilament triplet L protein; NFL
  • Application(s) IF, ICC, IHC, WB
  • Antibody Host Chicken
  • Antibody Type Polyclonal
  • Specificity Species cross-reactivity includes human, rat, mouse, cow and pig. Predicted to react with other mammalian tissues due to sequence homology.
  • Species Reactivity Bovine, Horse, Human, Mouse, Pig, Rat
  • Immunogen Description The antibody has been made against a preparation of recombinant full length human NF-L. The antibody binds NF-L from a variety of species including human, rat and mouse.
  • Conjugate Unconjugated
  • Purity Description IgY Preparation
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. The cultures were stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb (red), and co-stained with product R-1388-50, Neurofilament heavy polypeptide phosphorylated (pNF-H), Rabbit pAb, (green). The Neurofilament Light (NF-L) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells. In contrast, the phosphorylated Neurofilament Heavy (NF-H) has a much more restricted expression pattern, being found only in developed axonal neurofilaments. Since both proteins are found in neurofilaments, the red and green patterns overlap, so that neurofilaments containing NF-L and phosphorylated NF-H appear yellowish. In contrast neurofilaments containing only NF-L appear red.

  • Analysis by western blot of NF-L expression in different tissue lysates probed with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb, (green, 1:20,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: cow spinal cord. Note NF-L proteins are known to have slightly different apparent SDS-PAGE molecular weights across species.

    Image of a rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1390-50, Neurofilament light polypeptide (NF-L), Chicken pAb, (green, 1:2,000), and co-stained with product M-377-100, NeuN/Fox3, Clone 1B7, Mouse mAb, (red, 1:5000). IHC Method: Following transcardial perfusion of rat with 4% PFA, brain was post fixed for 24 hours, cut to 45 uM, and free-floating sections were stained. The NF-L antibody stains perikarya and processes of neuronal cells, particularly strongly the axons of basket cells, while product M-377-100 stains the nuclei and proximal cytoplasm of neurons.

    Citations & References

  • Specific References Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91.