Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (6H112)

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    Product Info

  • Product Name Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (6H112)
  • Product Description google Mouse anti-Neurofilament light polypeptide (NF-L), Monoclonal Antibody (Unconjugated), Clone 6H112, suitable for WB and Immunostaining.
  • Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL
  • Application(s) IF, ICC, IHC, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Species cross-reactivity includes human, rat, mouse, cow, and pig.
  • Species Reactivity Bovine, Human, Mouse, Pig, Rat
  • Immunogen Description The antibody has been made against the C-terminal peptide of human NF-L protein (amino acids 514-542; peptide GEEEDTKESEEEEKKEESAGEEQVAKKKD) with an N-terminal C added for coupling to KLH.
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image of a rat frontal cortex by Immunofluorescence and Immunohistochemistry. The section was stained with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:2000), and co-stained with product R-3771-100, FOX3/NeuN, Rabbit pAb, (red, 1:2,000). Method: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels cell bodies and processes of pyramidal neurons,dendrites, and axons of other neuronal cells. The FOX3/NeuN antibody selectively stains nuclei and cytoplasm of neuronal cells.

  • Analysis by western blot of NF-L expression in spinal cord lysates from different species with M-2114-50, Neurofilament light polypeptide (NF-L), Clone 6H112, Mouse mAb, (green, 1:5,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat; Lane 3: mouse; Lane 4: pig; Lane 5: cow.

    Citations & References