Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (7D1)

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    Product Info

  • Product Name Neurofilament light polypeptide (NF-L), Mouse Monoclonal Antibody (7D1)
  • Product Description google Mouse anti-Neurofilament light polypeptide (NF-L), Monoclonal Antibody (Unconjugated), Clone 7D1, suitable for WB, Immunostaining (ICC/IF)
  • Alternative Names NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL
  • Application(s) IF, ICC, WB
  • Antibody Host Mouse
  • Antibody Type Monoclonal
  • Specificity Species cross-reactivity includes human, rat, mouse, cow, pig and horse.
  • Species Reactivity Bovine, Horse, Human, Mouse, Pig, Rat
  • Immunogen Description The antibody has been made against a preparation of recombinant full length human NF-L; native protein purified from pig spinal cord. The antibody binds NF-L from a variety of species including human, rat and mouse.
  • Conjugate Unconjugated
  • Purity Description Protein G purified
  • Regulatory Status For research use only.

    Images, Protocols & SDS

  • Image of a mouse cerebellum by Immunofluorescence. The section was stained with M-2112-50, Neurofilament light polypeptide (NF-L), Clone 7D1, Mouse mAb, (green, 1:5000), and co-stained with product chicken antibody to FOX2, (red, 1:2,000) IF Method: Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post-fixed for 24 hours, cut into 45 µm sections, and free-floating sections were stained. The NF-L antibody labels dendrites and axons of neuronal cells, predominantly axons in white matter and basket cell axons associated with Purkinje cells. The FOX2 antibody reveals protein expressed in a subset of neurons, including Purkinje cells.

  • Analysis by western blot of NF-L expression in different tissue lysates with M-2112-50, Neurofilament light polypeptide (NF-L), Clone 7D1, Mouse mAb, (green, 1:5,000) with strong banding at 68-70kDa corresponding to the NF-L protein. Lane 1: protein standard (red); Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord.

    Citations & References