Product NameNeurofilament medium polypeptide (NF-M), Chicken Polyclonal Antibody
Product DescriptiongoogleChicken anti-Neurofilament medium polypeptide (NF-M), Polyclonal Antibody (Unconjugated), suitable for WB and Immunostaining.
Alternative NamesNF-M; NFM; Neurofilament medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm
Application(s)IF, ICC, IHC, WB
Antibody HostChicken
Antibody TypePolyclonal
SpecificitySpecies cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology.
Species ReactivityBovine, Chicken, Horse, Human, Mouse, Other Mammals (Predicted), Pig, Rat
Immunogen DescriptionThis antibody has been made against a recombinant fusion protein containing the extreme C-terminus of human NF-M (amino acids 708-877) expressed in and purified from E. coli.
Application DetailsWestern blot (WB), Immunocytochemistry (ICC) / Immunofluorescence (IF) and Immunohistochemistry (IHC). A dilution of 1:2,000 - 1:5,000 is recommended for WB. A dilution of 1:2,000 is recommended for ICC/IF and IHC. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeurofilament medium polypeptide (NF-M)
SpecificitySpecies cross-reactivity includes human, rat, mouse, cow, pig, horse and chicken. Band appears at ~145 kDa for rodent and ~160 kDa for human and cow in WB. Predicted to react with other mammalian tissues due to sequence homology.
Target Host SpeciesHuman
Species ReactivityBovine, Chicken, Horse, Human, Mouse, Other Mammals (Predicted), Pig, Rat
Antibody HostChicken
Antibody TypePolyclonal
Antibody IsotypeIgY
ConjugateUnconjugated
Immunogen DescriptionThis antibody has been made against a recombinant fusion protein containing the extreme C-terminus of human NF-M (amino acids 708-877) expressed in and purified from E. coli.
Purity DescriptionIgY Preparation
FormatLyophilized from PBS buffer pH 7.2-7.6 with 5mM sodium azide.
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute with 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsStore lyophilized antibody at 2-8°C After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles. Store at 4°C for up to one month for short term storage and frequent use.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNF-M; NFM; Neurofilament medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein; Nefm; Nef3; Nfm
Scientific BackgroundNeurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H, though other filament proteins may be included also. The major function of neurofilaments is likely to control the diameter of large axons. NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at 68-70kDa with some variability across species. Antibodies to NF-L are useful for identifying neuronal cells and their processes in cell culture and sectioned material. NF-L antibody can also be useful for the visualization of neurofilament rich accumulations seen in many neurological diseases, such as Lou Gehrig’s disease (ALS), giant axon neuropathy, Charcot-Marie Tooth disease and others. (Ref: uniprot.org)
Image of mixed neuron and glial cultures stained by Immunofluorescence and Immunocytochemistry. These cultures were stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red). The Neurofilament Medium (NF-M) protein is assembled into neurofilaments which are found throughout the axons, dendrites and perikarya of these cells.
Image of rat cerebellum section by Immunofluorescence and Immunohistochemistry. The section was stained with C-1393-50, Neurofilament medium polypeptide (NF-M), Chicken pAb, (red, 1:1,000) and co-stained with product M-1802-50, 2',3'-cyclic-nucleotide 3'-phosphodiesterase (CNP), Mouse mAb (green, 1:500). The blue is DAPI staining of nuclear DNA. Method: Following transcardial perfusion of rat with 4% PFA, brain was post-fixed for 24 hours, cut to 45 uM, and free-floating section was stained. The NF-M antibody labels the network of axons of basket neurons and other neurons. The CNP antibody stains oligodendrocytes, cells that create myelin sheaths around axons.
Analysis by western blot of NF-M expression in neuronal tissue and cell lysates with C-1393-50 (green, 1:2000) with strong bands at 145kDa (rodent NF-M molecules) and 160 kDa (human NF-M protein, visible in SHSY-5Y and HEK293 cells which have neuronal properties.) Lane 1:protein standard; Lane 2: rat brain; Lane 3: rat spinal cord; Lane 4: mouse brain; Lane 5: mouse spinal cord; Lane 6: NIH/3T3 cells; Lane 7: HEK293; Lane 8: HeLa; Lane 9: SH-SY5Y; Lane 10: C6 cells. NF-M is not expressed in HeLa and other cell lines tested.
Specific ReferencesJarjour A.A. et al (2007) Maintenance of axo-oligodendroglial paranodal junctions requires DCC and netrin-1. J Neurosci. 2008 Oct 22;28(43):11003-14. Rangaraju S. et al (2009) Molecular architecture of myelinated peripheral nerves is supported by calorie restriction with aging. Aging Cell. 2009 Apr;8(2):178-91. Pearse D.D. et al (2007) Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery. Glia. 2007 Jul;55(9):976-1000. Shaw G. et al (2004) Characterization of the bovine neurofilament NF-M protein and cDNA sequence, and identification of in vitro and in vivo calpain cleavage sites. Biochem Biophys Res Commun. 2004 Dec 10;325(2):619-25.
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