Alternative NamesNeuropeptide NPVF; RFRP; Neuropeptide VF; C7orf9; FMRFamide-related peptides;
Application(s)IHC-Frozen
Antibody HostGuinea Pig
Antibody TypePolyclonal
SpecificitySpecificity was demonstrated by immunohistochemistry. This antibody is known to react with rat and sheep.
Species ReactivityHuman, Rat, Sheep
Immunogen DescriptionA synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response.
Application DetailsImmunohistochemistry (IHC). A concentration of 1 in 2000 is recommended. IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells and terminals. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.
TargetNeuropeptide RFRP-3
SpecificitySpecificity was demonstrated by immunohistochemistry. This antibody is known to react with rat and sheep.
Target Host SpeciesHuman
Species ReactivityHuman, Rat, Sheep
Antibody HostGuinea Pig
Antibody TypePolyclonal
Antibody IsotypeMixed
ConjugateUnconjugated
Immunogen DescriptionA synthetic peptide (VPNLPQRF) corresponding to the amino acids 124-131 from human Neuropeptide VF. Neuropeptide VF is the precursor of the neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3. The synthetic peptide was conjugated to a carrier protein KLH to enhance the immunological response.
Purity DescriptionNeat serum
FormatLyophilized
Reconstitution InstructionsSpin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material.
Storage InstructionsAt least 12 months after purchase at 2-8°C (lyophilized formulations). After reconstitution, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.
Batch NumberPlease see item label.
Expiration Date12 months after date of receipt (unopened vial).
Alternative NamesNeuropeptide NPVF; RFRP; Neuropeptide VF; C7orf9; FMRFamide-related peptides;
Scientific BackgroundNeuropeptide VF is the precursor of neuropeptides NPSF (RFRP-1), RFRP-2 and RFRP-3 (NPVF). RFRP-3 is reported to inhibit forskolin-induced production of cAMP. RFRP-3 has also been shown to block morphine-induced analgesia.
Immunoreactivity of cells and terminals in sheep hypothalamus section using anti-RFRP-3 antibody (1:2000).
Staining of RFRP-3 peptide is not affected by preadsorption with Kisspeptin.
Staining of RFRP-3 peptide is not affected by preadsorption with Prolactin-releasing peptide (PrRP).
Staining of RFRP-3 peptide is not affected by preadsorption with NFF peptide.
Immunohistochemical detection of RFRP-3 with the guinea pig polyclonal antiserum (gp-1080-50; 1: 40,000), followed by detection with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen, produces specific labeling of RFRP-3/GnIH neurons and their processes in the dorsomedial nucleus of the rat hypothalamus. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.
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