Neurotrophin-4/5 (NT-4/5), Human, Mouse, Rat, Monkey, Rapid™ ELISA assay

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Catalog Number
BEK-2218

    Product Info

  • Product Name Neurotrophin-4/5 (NT-4/5), Human, Mouse, Rat, Monkey, Rapid™ ELISA assay
  • Product Description google
    The Biosensis Neurotrophin 4/5 RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-003-1000).

    This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards.

    This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.

    The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5.

    This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
  • Alternative Names NT4; NT5; NT4/5; Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4; Neurotrophin 4/5; NT 4/5
  • Application(s) ELISA
  • Specificity Human. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
  • Species Reactivity Human, Mouse, Primate, Rat
  • Immunogen Description Recombinant human NT4/5, made in E.coli
  • Range 15.6 - 1,000 pg/mL
  • Sensitivity This ELISA kit typically detects down to 25 pg/mL human NT4/5 (defined as 150% of blank value).
  • Regulatory Status For research use only.

    Specifications

  • Product Description
    The Biosensis Neurotrophin 4/5 RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the specific, fast and reliable quantification of NT4/5 in less than 4 hours in cell culture supernatants, human citrate-plasma and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular blood samples. Accurate quantification of NT4/5 in human citrate-plasma requires a heterophilic antibody (HA) blocker which can be purchased separately (BL-003-1000).

    This ELISA kit consists of a pre-coated polyclonal anti-NT4/5 capture antibody, a biotinylated anti-NT4/5 detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3',5,5'-tetramethylbenzidine, TMB) yields a colored reaction product, which is directly proportional to the concentration of NT4/5 present in samples and protein standards.

    This NT4/5 ELISA kit employs a recombinant human NT4/5 standard. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.

    The antibodies used in this kit bind to epitopes within the mature domain of NT4/5. Thus, this ELISA kit will quantify the mature form of NT4/5, and may also detect the full-length pro-form of NT4/5.

    This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.
  • Related Products Heterophilic antibody blocker for BEK-2226 & BEK-2218 and similar ELISA assays
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Neurotrophin-4/5 (NT-4/5) in Culture Supernatant, Plasma (Citrate), Tissue Homogenates. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Neurotrophin-4/5 (NT-4/5)
  • Specificity Human. The capture and detection antibodies will also detect NT4/5 from other species due to a high degree of NT4/5 amino acid sequence homology. Therefore, this ELISA kit can be used to quantify NT4/5 in many species including mouse, rat and monkey.
  • Target Host Species Human
  • Species Reactivity Human, Mouse, Primate, Rat
  • Immunogen Description Recombinant human NT4/5, made in E.coli
  • Range 15.6 - 1,000 pg/mL
  • Sensitivity This ELISA kit typically detects down to 25 pg/mL human NT4/5 (defined as 150% of blank value).
  • Sample Type Culture Supernatant, Plasma (Citrate), Tissue Homogenates
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plates, protein standards, detection reagents, wash and sample buffers, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C.
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date 12 months from purchase.
  • Alternative Names NT4; NT5; NT4/5; Neurotrophin-5; NT-5; Neutrophic factor 5; Neurotrophin-4; NT-4; Neutrophic factor 4; NTF5; NTF4; Neurotrophin 4/5; NT 4/5
  • Uniprot Number P34130
  • Uniprot Number/Name P34130 (NTF4_HUMAN)
  • Scientific Background FUNCTION: Target-derived survival factor for peripheral sensory sympathetic neurons. SUBCELLULAR LOCATION: Secreted protein. TISSUE SPECIFICITY: Highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. Expressed in embryonic and adult tissues. SIMILARITY: Belongs to the NGF-beta family.
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References

  • Specific References

    Kania K et al. (2024) Predicting clinical progression and cognitive decline in patients with relapsing-remitting multiple sclerosis: a 6-year follow-up study  Neurol. Neurochir. Pol. Ahead of Print Application: Human Peripheral Blood

    Loch AA et al. (2023). Plasma levels of neurotrophin 4/5, NGF and pro-BDNF influence transition to mental disorders in a sample of individuals at ultra-high risk for psychosis Psychiatry Res. [Epub ahead of print]. Application: Human plasma (EDTA).

    March B et al. (2021) ELISA-based quantification of neurotrophic growth factors in urine from prostate cancer patients. FASEB Bioadv. [Epub ahead of print]. Application: Human urine.

    Ishimoto T et al. (2018) Ergothioneine-induced neuronal differentiation is mediated through activation of S6K1 and neurotrophin 4/5-TrkB signaling in murine neural stem cells. Cell Signal. [Epub ahead of print]. Application: Mouse cell lysate and brain homogenate.

    Allen RS et al. (2018) TrkB signaling pathway mediates the protective effects of exercise in the diabetic rat retina. Eur J Neurosci. doi: 10.1111/ejn.13909. [Epub ahead of print]. Application: Rat retina homogenates.

    Maejima H et al. (2017) Exercise enhances cognitive function and neurotrophin expression in the hippocampus accompanied by changes in epigenetic programming in senescence-accelerated mice. Neurosci Lett. doi: 10.1016/j.neulet.2017.11.023. [Epub ahead of print]. Application: Mouse hippocampus homogenates.

    Takahashi K et al. (2016) Exercise combined with low-level GABAA receptor inhibition up-regulates the expression of neurotrophins in the motor cortex. Neurosci Lett. doi: 10.1016/j.neulet.2016.10.052. [Epub ahead of print]. Application: Mouse cortex homogenates, in native cell lysis buffer.

  • General References Lodouichi (2000) J. Neuroscie. 20(6): 2155
    Zhang et al. (1999) J Neurosci. Meths. 89 (1): 69