Neutrophil gelatinase-associated lipocalin-2 (NGAL), Human, ELISA assay

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Catalog Number
BEK-2141

    Product Info

  • Product Name Neutrophil gelatinase-associated lipocalin-2 (NGAL), Human, ELISA assay
  • Product Description google The human Lipocalin-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Lipocalin-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Lipocalin-2 concentration are added to these wells and allowed to complex with the bound Lipocalin-2 antibody. A biotinylated human Lipocalin-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Lipocalin-2 in samples such as culture supernatant, serum, plasma (Heparin), saliva and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Alternative Names Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; p25; LCN2; HNL;
  • Application(s) ELISA
  • Specificity Human Lipocalin-2/NGAL
  • Species Reactivity Human
  • Immunogen Description The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant human Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system.
  • Range 156 pg/mL - 10,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Regulatory Status For research use only.

    Specifications

  • Product Description The human Lipocalin-2 Kit is a sandwich ELISA. The capture antibody is a polyclonal human Lipocalin-2 antibody pre-coated onto the 96-well strip plates provided in the kit. Human test samples and standards of known Lipocalin-2 concentration are added to these wells and allowed to complex with the bound Lipocalin-2 antibody. A biotinylated human Lipocalin-2 polyclonal antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Lipocalin-2 present in the samples. The purpose of this kit is the in-vitro quantitative determination of human Lipocalin-2 in samples such as culture supernatant, serum, plasma (Heparin), saliva and urine. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
  • Application(s) ELISA
  • Application Details ELISA. For the quantification of Neutrophil gelatinase-associated lipocalin-2 (NGAL) in Culture Supernatant, Serum, Plasma (Heparin), Saliva, Urine. Please download the detailed product insert for complete instructions for the successful use of this ELISA. Use only as directed.
  • Target Neutrophil gelatinase-associated lipocalin-2 (NGAL)
  • Specificity Human Lipocalin-2/NGAL
  • Target Cross-Reactivity (ELISA) There is no detectable cross-reactivity with other relevant proteins.
  • Target Host Species Human
  • Species Reactivity Human
  • Immunogen Description The standard and antigen for this Lipocalin-2/NGAL ELISA is recombinant human Lipocalin-2/NGAL amino acids 21-N198 produced in an purified from a mammalian cell expression system.
  • Range 156 pg/mL - 10,000 pg/mL
  • Sensitivity < 10 pg/mL
  • Sample Type Culture Supernatant, Plasma (Heparin), Saliva, Serum, Urine
  • Detection Method Colorimetric
  • Kit Components The ELISA kit box contains 96-well pre-coated strip plate(s), protein standards, detection reagents, substrate buffer and detailed protocols.
  • Storage Instructions Store at 2-8°C
  • Storage Temperature 2-8°C
  • Batch Number Please see item label.
  • Expiration Date This kit may be stored for up to 6 months at 2°C to 8°C from the date of shipment. Standards should be stored at -20°C or -80°C (recommended at –80°C) after reconstitution. Coated strips or reagents may be store for up to 1 month at 2°C to 8°C.
  • Alternative Names Neutrophil gelatinase-associated lipocalin; NGAL; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3; p25; LCN2; HNL;
  • Uniprot Number P80188
  • Uniprot Number/Name P80188 (NGAL_HUMAN)
  • Shipping Statement For order quantities larger than 1 plate, this product is supplied in package units of 2 plates.
  • Shipping Temperature 2-8°C (on cold packs)
  • UNSPSC CODE 41116158
  • Regulatory Status For research use only.

    Citations & References